FHC Human Colon Epithelial Cells

The cell line originated from a 13 week old male fetus. The cells presented epithelial morphology, but no cytoplasmic keratin was detected. It has been identified as free of mycoplasma/STR and put into storage.

Animal Species/Organism Boys

Tissue and Cell Type Small intestine, epithelial cells

Morphology Epithelial cell like, adherent growth

Medium and Culture Conditions:

Complete culture medium: low serum epithelial cell culture medium (product number: P9001)

Freeze storage conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: non programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is only for scientific research use

PubMed=6519668; DOI=10.1007/BF02619632

Siddiqui K.M., Chopra D.P.

Primary and long term epithelial cell cultures from human fetal normal colonic mucosa.

In Vitro 20:859-868(1984)

PubMed=20193843; DOI=10.1016/j.cancergencyto.2009.11.009

Soucek K., Gajduskova P., Brazdova M., Hyzd'alova M., Koci L., Vydra D., Trojanec R., Pernicova Z., Lentvorska L., Hajduch M., Hofmanova J., Kozubik A.

Fetal colon cell line FHC exhibits tumorigenic phenotype, complex karyotype, and TP53 gene mutation.

Cancer Genet. Cytogenet. 197:107-116(2010)