No Programmed Cryopreservation Cells Solution (low Serum)

It is recommended to use cell seed preservation and primary cells. The serum-containing cryopreservation solution is prepared with cell-derived growth factor, trehalose, propylene glycol and a small amount of DMSO and FBS as the main raw materials. It is suitable for the cryopreservation of various precious mammalian primary cells, cell lines and hybridoma cells. It contains a small amount of protein components to maintain the nutrition needed for self-repair of cells during freezing and dormancy. This product is a ready-to-use reagent and is stable in storage, Easy to use and high cell survival rate. The cells frozen with this product need not be cooled in the process of freezing, and can be directly placed at - 80 ℃. There is no need for centrifugation during resuscitation, and the fluid can be changed within 12-16 hours after resuscitation.

matters needing attention:

1. After initial use and melting, it can be stored at 4 ℃ for 1 month, but the number of repeated freezing and thawing should be reduced to avoid failure.

2. It is best to freeze cells in logarithmic growth phase.

3. Control the digestion time of pancreatin, and remember that excessive digestion will affect the effect of cell resuscitation.

4. During the process of cell resuspension, please be gentle to avoid damaging the cell, but at the same time, it must be fully resuspended, so that the cell will not form a cluster during the recovery.

5. Please pay attention to sterility during cell operation.

6. For your safety and health, please wear lab clothes and disposable gloves.

Transportation and storage:

Dry ice and Ice bag transportation, stored at - 20 ℃

Statement: The product is for scientific research only