At a glance: Here are the steps for the BBB experiment

BBB experiment is enduring, but in the actual operation is very easy to leak, take a look at the following steps to build it, all dry goods to share, a point of attention, the way of scientific research is not lost

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Inthe Protocol of neuronal cells in one step

Macro-spectrum: Can MSCs also induce neuronal adult cells? Protocol Our technology is sorted out, waiting for you to read!

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The pitfalls of digestive organoids over the years

Organoid culture is a method of using stem cell technology to simulate the development process of organs in the human body and construct a three-dimensional cell culture model with similar organ structure and function. Such models can help scientists better understand organ development, disease mechanisms and the effects of drugs. Organoids are generally purified through induction and intervention, and passage is also an indispensable step in cultivating organoids;

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Is your culture bottle wrapped?

When we buy culture bottles, we know that TC-treated culture plates are suitable for adherent cells, because the cells are attached quickly and grow well, while we use non-TC-treated boards for suspended cells, because the surface of the non-TC-treated culture plates is rough, and suspended cells do not need to attach walls, which will grow better.

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Hybridoma cells 1.2.3.4

Hybridoma cells are a special type of cell that is formed from a fusion of cancer cells and immune cells (such as B cells) outside the body. Hybridoma cells have the ability of infinite proliferation of cancer cells and antigen recognition of immune cells, so they are widely used in biomedical research and production. However, the screening of hybridoma cells is a very critical step, and only the appropriate hybridoma cells can be selected to ensure the accuracy and reliability of subsequent experiments.

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There are many kinds of cell aging tests, depending on which one you choose, let's take a look at mine

Cellular senescence research is of great significance for understanding the aging process of organisms and preventing and treating age-related diseases. By studying the mechanisms of cellular aging, we can reveal the molecular and genetic factors that lead to decreased cell function and tissue degeneration. This could help develop new drugs and treatments to slow the aging process and improve the quality of life of older people. In addition, cell aging research can also provide a theoretical basis for regenerative medicine and tissue engineering, and promote the development of related fields

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My history with the CCK-8 experiment

CCK-8 test, or Cell Counting Kit-8 test, is a widely used experimental method for cell proliferation and cytotoxicity detection. This method originated from the need for cell proliferation and toxicity analysis, aiming to provide a simple, accurate and highly sensitive assay.

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How to choose between G418 and puromycin in cell screening?

There are significant differences between G418 and purinomycin in chemical properties, mechanism of action and application in biological studies.

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Interesting history of cloning

Lost in all the buzz about cloning is the fact that cloning is nothing new: its rich scientific history spans more than 100 years. The following landmark examples will take you on a journey through time, where you can learn more about the history of cloning.

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Summary of true criteria for lipid classification and identification

Lipid research is a broad and in-depth field, involving medicine, biology, chemistry and other disciplines, and a large number of research papers are published every year

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When cells encounter mycoplasma? Don't worry. This is it. Mycoplasma is gone

Mycoplasma (PPLO) is a small (0.2-0.3 micron) mencyless bacterium that can grow to very high concentrations, typically 107-108 organisms /ml, in mammalian cell cultures that are still not visible through conventional light microscopy

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It's all cleaned up, to avoid the pits where the primary cells are easy to step on

Primary cell culture seems to be a simple problem, but it is actually found that we really need to sum up these tips of primary culture after hundreds of experiments

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How to extract the little secret of melanocytes

Experimental dogs always have to do the experiment, this is not, just do the week cells, melanocytes and climb the mountains and wade in the rush to come over, pick up surgical scissors, forceps and other experimental small helper began to operate it!

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The operation of cell phagocytosis experiment, the "Hunger games" of cells, was described

Cytophagocytosis is the function of some specific cells in the organism to recognize foreign bodies and swallow and destroy them.

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Master the following 9 tips to easily isolate and culture epidermal keratinocytes

The separation steps of epidermal keratinocytes mainly include the following steps: 1. Take a 1.5mm thick skin sheet and (preferably not more than 12 hours) rinse the obtained skin material with a mixture of 4℃MEM and HBSS for 2-3 times. The ice bath is done in a petri dish. The first time, the blood is flushed away, the second time, the remaining tissue is scraped away, leaving the dermis and epidermis, and the tissue is transferred to a new dish after the first rinse 2. Smooth the skin, cut it into 2-3mm² pieces with a scalpel, and put it into a neutral protease digestion solution for 15-20h at 4℃, or 2-4h at 37℃. 3. Separate dermis and epidermis with sterile needles and ophthalmic tweezers. The epidermis is thin and transparent, and the dermis is thick and slightly swollen and gelatinous under digestion by pancreatic enzymes. 4. The isolated epidermal blocks were digested with 0.25% trypsin and oscillated at 37℃ for 10-30min until the epidermis was organized into flocculent form, and then culture medium containing 10% fetal bovine serum was added to terminate digestion.

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