STR identification description:
9 /16 STR loci were used to type the cell samples by fluorescence labeling amplification product length polymorphism analysis. PCR primers were designed and two panels were formed for expanding polymorphic sites. 5 'FAM was used as the fluorescent label on the primer. Primers used online Primer3 software design (http://frodo.wi.mit.edu/cgi-bin/primer3/primer3_www.cgi). A small amount of PCR products were diluted and mixed with internal label, and then directly applied to ABI 3130xl for capillary electrophoresis. Data files were analyzed using GeneMapper4.0 (Appliedbiosystems).
STR (Short Tandem Repeat) is a class of DNA tandem repeats widely existing in eukaryotic genomes. Due to individual differences in the number of repeats of its core sequence, STR is also known as the DNA fingerprint of cells. In 2011, the United States issued the National Standard for cell STR Identification (ANSI/ATCC ASN-0002-2011). STR test has been widely used in paternity identification, individual identification, cell origin determination and so on. International well-known cell banks such as ATCC, DSMZ and JCRB have listed STR genotyping as the gold standard for cell identification.
Requirements for cell sample delivery:
The cell suspension of more than 1×10 to the fifth power was placed in a 1.5ml EP tube, sealed with a sealing film, and the cell name (such as HepG2) was clearly marked on the tube wall. If there was a large number of cells, it could be numbered, and the test list was attached.
At the same time, please send the following information to our online customer service: name of the company (invoice title), name of the person in charge of the project (boss), telephone number and email address. The company will send the contract to the customer, and the person in charge will sign the contract and express it to the company together with the cell suspension.