The cell biology technology platform systematically carries out basic and applied research in the field of life science at the cellular level. It is equipped with an experimental environment of overall 10,000 level and local 100 level clean areas, and is equipped with advanced instruments such as biosafety cabinet, cell counter, CO2 incubator, bioinverted microscope, liquid nitrogen storage tank, BD flow cytometry analyzer, MD automatic high-content imaging analysis system, etc. The main services include cell culture, apoptosis detection, cell proliferation and toxicity, gene overexpression/silencing, cell metastasis research, flow cytometry detection, cell identification, automatic high connotation imaging analysis system detection, etc.
Cell biology platform
| Experimental name | Experimental content | Customer provided materials | Term of service | Service quote |
| Isolation and identification of primary cells | Cell isolation and culture | Specific components in tissue and culture medium | 2 weeks | Make an inquiry |
| Passage cell culture (dosing intervention) | Resuscitation, passage and culture | Cell and culture conditions | 2 weeks | Make an inquiry |
| Construction of drug-resistant strains in tumor cells | Construction of drug-resistant cell lines | Cell and drug | In June | 10000 |
| Nuclear extraction | Nuclear extraction | cell | 1 week | 100 |
| Mitochondrial extraction | Mitochondrial extraction | cell | 1 week | 150 yuan/sample |
| Transient transfection | Transient transfection | DNA, small oligonucleotides, or siRNA | 2 weeks | 2000 yuan per sample |
Stable transfection (Stable cell line construction) | Stable transfection | DNA, small oligonucleotides, or siRNA | February | 9600 yuan per sample |
| Adenovirus packaging and infection | The shuttle vector with the target fragment reacts with the virus skeleton vector in LR | With target fragment interference vector | 1 week | Make an inquiry |
| Linearized and transfected | cell | 1 week | ||
| Collecting adenovirus | cell | 1 week | ||
| Lentivirus packaging and infection | The DNA incubates with the liposome | With target fragment interference vector | 1 week | Make an inquiry |
| Cell culture | cell | 1 week | ||
| Harvest virus | virus | 1 week | Make an inquiry | |
| RNAi | RNAi | Target gene sequence | 4-8 weeks | Make an inquiry |
| MicroRNA | MicroRNA | Target gene sequence | 4-8 weeks | Make an inquiry |
Luciferase test/pair Luciferase reporter gene | Reporter gene test | plasmid | 4 weeks | 12000/ pair |
| MTT/CCK-8 detection | MTT | Drugs, cells | 3 weeks | 800 yuan/medicine/cell |
| CCK-8 | Drugs, cells | 3 weeks | 900 yuan/medicine/cell | |
| Growth curve/survival curve measurement | Growth curve measurement/survival curve measurement | Cells and culture conditions | 2 weeks | 1000 yuan per plant |
Apoptosis detection (Annexin V/PI double stain) | Flow detection | cell | 1 week | 100 yuan per sample |
| Cell cycle detection | Cell cycle detection | cell | 3 days | 120 yuan/sample |
| Clonal formation | Clonal formation | Cells, drugs | 3 weeks | 1000 yuan per 6 holes |
| edu labeling | pretest | A section of cells or tissue | 2 to 4 weeks | 500 yuan |
| Flow detection | 100 yuan/sample | |||
| Immunohistochemical test | 100 yuan/sample | |||
| Cell invasion | Transwell was used to detect cell invasion | Cells (Transwell chamber, matrix glue, FN) | 5 days | 500 yuan per sample |
| Cell migration | Transwell was used to detect cell migration | Cells (Transwell chamber, matrix glue, FN) | 5 days | 300 yuan/sample |
| Cell adhesion | Cell adhesion was detected by MTT assay | Cells (Transwell chamber, matrix glue, FN) | 5 days | 1000 yuan per sample |
| Cell scratch | Cell scratch experiment | Cell and culture conditions | 5 days | 100 yuan per sample |
| Cell coculture | Cell coculture | Cells (Transwell chamber, matrix glue, FN) | 5 days | 300 yuan/sample |
| Cell chemotaxis | Transwell was used to detect cellular chemotaxis | Cells (Transwell chamber, matrix glue, FN) | 5 days | 300 yuan/sample |
Annexin V-FITC /EGFP/PE/APC | Annexin V single inspection | Cells (1×105 cells/experiment) | 3 days | 120 yuan per sample |
| 300 yuan/sample | ||||
| Hoechst 3342/33258 Dyed | Cell staining | cell | 3 days | 100 yuan/sample |
PI/DAPI/EB/ 7-AAD/ Rhodamine 123 / acridine orange staining | Cell staining | cell | 3 days | 100 yuan/sample |
| In situ detection of Tunel apoptosis | pretest | Cell smear, tissue frozen section, tissue paraffin section | 2 weeks | 500 yuan/set (including negative and positive control) |
| TUNEL single experiment | Cell smear, tissue frozen section, tissue paraffin section | 2 to 4 weeks | 200 yuan/sample | |
| Mitochondrial membrane potential (JC-1) | The JC-1 experiment | Cells (1×105-106 cells) | 3 days | 100 yuan per sample |
Regulatory T (Treg) Cell detection (CD4/ CD25/FOXP3) | Flow detection | Cells, detect antibodies | 1 week | 100 yuan per sample |
| T lymphocyte assay (CD3+) | Flow detection | Cells, detect antibodies | 1 week | 100 yuan per sample |
T helper cell (CD3+CD4+), Th1, Th2 | Flow detection | Cells, detect antibodies | 1 week | 100 yuan per sample |
T-suppressor cell (CD3+CD8+), Including Tc1, Tc2 | Flow detection | Cells, detect antibodies | 1 week | 100 yuan per sample |
NK cell assay (CD28/CD8) | Flow detection | Cells, detect antibodies | 1 week | 100 yuan per sample |
| B cell assay (CD19) | Flow detection | Cells, detect antibodies | 1 week | 100 yuan per sample |
Platelet granule membrane Glycoprotein detection (CD62p/CD63) | Flow detection | Cells, detect antibodies | 1 week | 100 yuan per sample |
| Determination of hematopoietic stem/Progenitor cells (CD34+) | Flow detection | Cells, detect antibodies | 1 week | 100 yuan per sample |
Dendritic cell detection (CMRF-44/56, CD40/80 etc.) | Flow detection | Cells, detect antibodies | 1 week | 100 yuan per sample |
Intracellular dissociation Ca2+ assay (Quin-2, Fluo-3, Indo-1) | Flow detection | Cell, kit | 1 week | 100 yuan per sample |
Intracellular PH was measured (DCH, BCECF, BCECF-AM) | Flow detection | Cell, kit | 1 week | 100 yuan per sample |
| Cell intima potential assay (JC-1) | Flow detection | cell | 1 week | 100 yuan per sample |
| Determination of intracellular REDOX status | Flow detection | Cell, kit | 1 week | 100 yuan per sample |
| Determination of reticulocyte | Flow detection | Cell, kit | 1 week | 100 yuan per sample |
| HLA-B27 typing | Flow detection | Cell, kit | 1 week | 100 yuan per sample |
| Cell mediated cytotoxicity experiments | Flow detection | cell | 1 week | 100 yuan per sample |
| Cell tracer detection | Flow detection | Cell, kit | 1 week | 100 yuan per sample |
Transplantation immunoassay (CD30/CD4/CD8) | Flow detection | Cells, detect antibodies | 1 week | 100 yuan per sample |
| Cell activation assay (CD3/4/25/69,CD14/HLA-DR) | Flow detection | Cells, detect antibodies | 1 week | 100 yuan per sample |
MSC detection (CD45 /106/34/44, etc.) | Flow detection | Cells, detect antibodies | 1 week | 100 yuan per sample |
| The detection of activated Caspase | Flow detection | Cells, detect antibodies | 1 week | 100 yuan per sample |
Multi-drug resistant eggs White determination (P-170) | Flow detection | Cells, detect antibodies | 1 week | 100 yuan per sample |
Phosphotyrosine excitation Determination of enzyme content (p-Tyr) | Flow detection | Cells, detect antibodies | 1 week | 100 yuan per sample |
Phosphotyrosine excitation Determination of Enzyme receptor FLT3 (STK1) | Flow detection | Cells, detect antibodies | 1 week | 100 yuan per sample |
| Cell proliferation assay (CFSE/Brdu) | Flow detection | Cells, detect antibodies | 1 week | 100 yuan per sample |
CIK cell immunity Phenotypic detection (CD3+CD56+) | Flow detection | Cells, detect antibodies | 1 week | 100 yuan per sample |
| Liver cell identification (CK-18/ALB) | Flow detection | Cells, detect antibodies | 1 week | 100 yuan per sample |
Peripheral blood, marrow plasma Cell detection (CD45/ CD38/CD138/CD19) | Flow detection | Cells, detect antibodies | 1 week | 100 yuan per sample |
PNH disease detection (CD55/CD59) | Flow detection | Cells, detect antibodies | 1 week | 100 yuan per sample |
Early hematopoietic stem Cell detection (CD34/CD38) | Flow detection | Cells, detect antibodies | 1 week | 100 yuan per sample |
Erythrocyte injury test (Annexin V) | Flow detection | cell | 1 week | 100 yuan per sample |
Cell activity detection (7-AAD) | Flow detection | cell | 1 week | 100 yuan per sample |
Platelet-associated antibody Body detection (IgG/IgA/IgM) | Flow detection | Cells, detect antibodies | 1 week | 100 yuan per sample |
CMV antigen detection (pp-65) | Flow detection | Cells, detect antibodies | 1 week | 100 yuan per sample |
Chronic lymphocyte white Blood disease test (ZAP-70) | Flow detection | Cells, detect antibodies | 1 week | 100 yuan per sample |
CD40 for coronary heart disease Volume detection (CD40L) | Flow detection | Cells, detect antibodies | 1 week | 100 yuan per sample |
Cytophagocytosis function test Measurement (DEX-FITC method) | Flow detection | Cell, kit | 1 week | 100 yuan per sample |
Neutrophil activation Detection (CD18/CD11b) | Flow detection | Cells, detect antibodies | 1 week | 100 yuan per sample |
Neutrophil respiratory burst Detection (DHR-123 method) | Flow detection | Cells, detect antibodies | 1 week | 100 yuan per sample |
Leukemic residual cell Detection (T-ALL cyto CD3/TdT,CD33 /TdT,CD7/TdT. | Flow detection | Cells, detect antibodies | 1 week | 100 yuan per sample |
| Detection of telomerase activity | TRAP-PCR silver staining method | 1~2×106 cells or 0.5g tissue | 2 weeks | 500 yuan per sample |
| Fluorescence quantitative PCR method | 1~2×106 cells or 0.5g tissue | 2 weeks | 600 yuan per sample |
