Organization Freezing Solution（XF)

The tissue cryopreservation solution is used for the preservation of tumor or normal tissue, and also for the freezing and transportation of organ-like organs. The cryopreservation solution has the advantages of long freezing time and high tumorigenesis rate. It does not contain serum and reduces serum pollution. The tissue cryopreservation solution is prepared according to the group distribution of tissue or organ-like organs during dormancy, which can meet the nutrient requirements of tissue or organ-like organs and tumor spheres during freezing, and antifreeze is added, It can reduce ice crystal damage during freezing.

instructions:

1.Take out and disinfect the tumor and cut it into 1 with sterile surgical scissors × one × 3m3 fragments (if the frozen ones are organoid or tumor spheres, please first Adjust the centrifuge to the speed of 2000RMP, place the sphere or similar organ that needs centrifugation, start the centrifuge, press the stop button after the speed reaches 2000, take out the centrifuge tube after the centrifuge stops completely, and then operate the second step)

2.Inhale the tissue or tumor sphere into the centrifuge tube and add the cryopreservation solution. The ratio of the tissue and cryopreservation solution is 1:1 (please freeze the frozen organs or tumor spheres according to the concentration of frozen cells).

3.Gently blow and put it in the prepared frozen storage tube, and use the program cooling box to cool down (put it in - 20 ° 12 hours and then put it in - 80 ° 24 hours before it can be transferred into liquid nitrogen)

Note: This product is only used for scientific research