Sprague Dawley (SD) Rat Bone Marrow MSC Cells Sprague Dawley (SD) Rat Bone Marrow Mesenchymal Stem Cells

Rat bone marrow mesenchymal stem cells, Cell volume 0.5×10⁶ per freezing tube. The bone marrow of Sprague Dawley (SD) rats taken from our library for 3-6 weeks was isolated and obtained by mechanical method in a sterile environment. SD rat mesenchymal stem cell complete culture medium (catalog number SCSP-615) (hereinafter referred to as MSC complete culture medium) was used for adherent culture, and passage amplification was conducted to the second generation (P2 generation) for frozen storage. Each batch passed the mycoplasma test in this library and the result was negative. This batch of cells has been tested to have the ability to differentiate into fat, osteogenesis, and cartilage. It is recommended to use cells before generation P5 for differentiation experiments.

Animal Species/Organism Rat, Sprague Dawley Strain.

Tissue and Cell Type Mouse Bone Marrow.

Morphology: Spindle, spindle, and polygonal, adherent growth.

Medium Formulation:

Complete mesenchymal stem cell culture medium (P2001)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37℃.

Freeze storage conditions: basic medium+5% DMSO+20% FBS

Complete cryopreservation solution: non programmed cooling cell cryopreservation solution (low serum) (SD0001)

Declaration: This product is for scientific research only.

Strategic targeting of miR-183 and β-catenin to enhance BMSC stemness in age-related osteoporosis therapy. Sci Rep 14, 21489 (2024). https://doi.org/10.1038/s41598-024-72474-0