3T6 Swiss Albino Mouse Embryonic Fibroblast

3T6 cell line was established in 1963 from decomposed Swiss mouse embryos and can secrete collagen and hyaluronic acid. The detection showed that the limb bone malformation virus (mouse pox) was negative. It was put into storage after identification without mycoplasma.

Animal species/Organism mice

Tissue and Cell Type embryos; Fibroblast

Morphology Fibroblast-like, adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

90% DMEM (MD0100)+10% fetal bovine serum (SD0200)+G/A (SD0038)

DMEM complete medium (article number: MD0101)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only

PubMed=13985244; DOI=10.1083/jcb.17.2.299

Todaro G.J., Green H.

Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines.

J. Cell Biol. 17:299-313(1963)

CLPUB00447

Mulivor R.A., Suchy S.F.

1992/1993 catalog of cell lines. NIGMS human genetic mutant cell repository. 16th edition. October 1992.

(In) Institute for Medical Research (Camden, N.J.) NIH 92-2011; pp.1-918; National Institutes of Health; Bethesda (1992)

DOI=10.3917/bhesv.191.0045

Barreiro E.W., Curras E.

Mutation carriers: led by Spaniards in U.S. laboratories in the early 80s.

Bull. Hist. Epistem. Sci. Vie 19:45-65(2012)