3T3-L1 Mouse Embryonic Fibroblast

L1 is a continuous sub-strain of 3T3 (swiss mouse) isolated by cloning. 3T3-l1 is a cell line derived from (mouse) 3T3 cells, which is used for biological research of adipose tissue. 3T3-L1 cells have fibroblast-like morphology, but under appropriate conditions, cells differentiate into adipocyte-like phenotype. 3T3-L1 cells with adipocyte morphology increase the synthesis and accumulation of triglycerides, and obtain the ring-like appearance of adipocytes. These cells are also sensitive to fat and lipid hormones and drugs, including epinephrine, isoproterenol and insulin. It was put into storage after identification without mycoplasma.

Tissue and Cell Type Embryos

Morphology Fibroblast-like, adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

90% DMEM (MD0100)+10% calf serum (SD0400)+G/A (SD0038)

DMEM complete medium (MD0101)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only

DOI=10.1016/0092-8674(74)90126-3

Green H., Kehinde O.

Sublines of mouse 3T3 cells that accumulate lipid.

Cell 1:113-116(1974)

PubMed=4426090; DOI=10.1016/0092-8674(74)90116-0

Green H., Meuth M.

An established pre-adipose cell line and its differentiation in culture.

Cell 3:127-133(1974)

PubMed=165899; DOI=10.1016/0092-8674(75)90087-2

Green H., Kehinde O.

An established preadipose cell line and its differentiation in culture. II. Factors affecting the adipose conversion.

Cell 5:19-27(1975)

Patent=US4003789

Green H.

Triglyceride-accumulating clonal cell line.

Patent number US4003789, 18-Jan-1977

PubMed=6698114; DOI=10.1016/0014-4827(84)90361-6

Levine J.F., Stockdale F.E.

3T3-L1 adipocytes promote the growth of mammary epithelium.

Exp. Cell Res. 151:112-122(1984)

PubMed=28176856; DOI=10.1038/srep42104

Kassotis C.D., Masse L., Kim S., Schlezinger J.J., Webster T.F., Stapleton H.M.

Characterization of adipogenic chemicals in three different cell culture systems: implications for reproducibility based on cell source and handling.

Sci. Rep. 7:42104-42104(2017)