MC3T3-E1 Subclone 14 Subclone 14 Of Mouse Preparietal Osteocytes

A series of subclones were isolated from MC3T3-E1 cell lines with different phenotypes. Select the subclones with high or low osteoblast differentiation and mineralization from the osteoblasts grown in the medium containing ascorbic acid. MC3T3 subclone 4 (ATCC CRL-2593) and MC3T3 subclone 14 (ATCC CRL-2594) showed high levels of osteoblast differentiation when grown in ascorbic acid and 3 to 4 mM inorganic phosphate. They form a well-mineralized extracellular matrix (ECM) after 10 days. MC3T3 subclone 24 (ATCC CRL-2595) and MC3T3 subclone 30 (ATCC CRL-2596) showed poor osteoblastic differentiation in ascorbic acid. Without ECM, it can be used as a negative control for subclones 4 and 14. Mineralized subclones selectively expressed mRNA as osteoblast markers, and mRNA of sialoglycoprotein (BSP), osteocalcin (OCN), and parathyroid hormone/parathyroid hormone-related protein receptor. Subclons with high or low differentiation potential produce similar amounts of collagen in culture, and express comparable basic level of mRNA encoding Osf2/Cbfa1, an osteoblast-related transcription factor. After implantation into the immunodeficient mice, the highly differentiated subclones form woven bone similar to bone, which forms small bones, while the poorly differentiated cells only produce fibrous tissue. These cell lines are good models for studying the differentiation of osteoblasts in vitro, especially ECM signals. Their behavior is similar to that of primary cultured cranial crest osteoblasts. Through mycoplasma detection in this library.

Animal species/Organism mice, NIH/Swiss

Tissue and Cell Type cranial parietal bone

Morphology Fibroblast-like, adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

80% α MEM (MD0800)+10% fetal bovine serum (SD0200)+inositol 43.2mg/L (SD0014)+folic acid 8.82mg/L (SD0016)+ β- Mercaptoethanol 7.8mg/L (SD0031)+G/A (SD0038)

Complete medium: α MEM complete medium (article number: MD0807)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only.

Nuclear-targeted carbon quantum dot mediated CRISPR/Cas9 delivery for fluorescence visualization and efficient editing†

Nanoscale, 2022,14, 14645-14660

https://pubs.rsc.org/en/content/articlelanding/2022/nr/d2nr04281a/unauth