C3H/10T1/2 Clone8 Mouse Embryonic Fibroblast

It was isolated from C3H mouse embryonic cells in 1972; No tumorigenesis was found in homologous mice and no spontaneous transformation was found; Chemicals can easily cause the cell

For transformation, it is recommended to only use cells of 5-15 generations. It was put into storage after identification without mycoplasma.

Animal species/Organism mice

Tissue and Cell Type Embryos

Morphology: Fibroblast-like, adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

90% DMEM (MD0100)+10% fetal bovine serum (SD0200)+G/A (SD0038)

DMEM complete medium (article number: MD0101)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only

PubMed=4357355

Reznikoff C.A., Brankow D.W., Heidelberger C.

Establishment and characterization of a cloned line of C3H mouse embryo cells sensitive to postconfluence inhibition of division.

Cancer Res. 33:3231-3238(1973)

PubMed=4796800

Reznikoff C.A., Bertram J.S., Brankow D.W., Heidelberger C.

Quantitative and qualitative studies of chemical transformation of cloned C3H mouse embryo cells sensitive to postconfluence inhibition of cell division.

Cancer Res. 33:3239-3249(1973)

PubMed=25277546; DOI=10.1186/1471-2164-15-847

Didion J.P., Buus R.J., Naghashfar Z., Threadgill D.W., Morse H.C. III, de Villena F.P.

SNP array profiling of mouse cell lines identifies their strains of origin and reveals cross-contamination and widespread aneuploidy.

BMC Genomics 15:847-847(2014)