Anti AChE Effect Of AE-2 Hybridoma Cells

Animals were immunized with purified human erythrocyte acetylcholinesterase. Spleen cells fused with gp2/O-Agl4 myeloma cells. The antibody produced binds to acetylcholinesterase of human and monkey, but the reaction site is different from the antigenic determinant of AE-1 (ATCC HB-72). Short limb deformity (mouse pox) virus was found to be negative. It was put into storage after identification without mycoplasma.

Animal species/Organism mouse B cell x mouse lymphoma

Tissue and Cell Type Lymphocytes, Lymphoma

Morphology lymphoblast-like, suspension growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

90% DMEM (MD0100)+10% fetal bovine serum (SD0200)+G/A (SD0038)

DMEM complete medium (article number: MD0101)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only

PubMed=6175961; DOI=10.1073/pnas.79.4.1078

Fambrough D.M., Engel A.G., Rosenberry T.L.

Acetylcholinesterase of human erythrocytes and neuromuscular junctions: homologies revealed by monoclonal antibodies.

Proc. Natl. Acad. Sci. U.S.A. 79:1078-1082(1982)