A9 Mouse Subcutaneous Connective Tissue Cells

A9 cell line was isolated from wild type cell line L. It is sensitive to HAT selective medium, and lacks adenosine phosphoribosylase and hypoxanthine phosphoribosylase, which are resistant to 8-azaguanine (0.003mg/ml) and 6-thioguanine (0.1mM). It was put into storage after identification without mycoplasma.

Animal species/Organism mice

Tissue and Cell Type subcutaneous connective tissue; Loose connective tissue and fat; normal

Morphology Fibroblast-like, adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

90% DMEM (MD0100)+10% fetal bovine serum (SD0200)+G/A (SD0038)

DMEM complete medium (article number: MD0101)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only

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