F9 Mouse Teratoma Cells

Under the stimulation of vitamin A acid and dibutyryl cAMP, F9 cells differentiated into the body wall endothelial layer. The differentiated cells synthesize plasma zymogen activator, laminin and type IV collagen. The cAMP on cells is activated only after treatment with vitamin A acid. There are three copies in the cell β 1 adhesin gene. The detection showed that the limb bone malformation virus (mouse pox) was negative.
Organizational source/Issue and Cell Type: Testis; Teratocellular carcinoma; Testicular teratocarcinoma
Morphology: Epithelial like, adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

90% DMEM (MD0100)+10% fetal bovine serum (SD0200)+G/A (SD0038)

DMEM complete medium (article number: MD0101)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only

PubMed=61803

Nicolas J.-F., Avner P., Gaillard J., Guenet J.-L., Jakob H., Jacob F.

Cell lines derived from teratocarcinomas.

Cancer Res. 36:4224-4231(1976)

PubMed=214238; DOI=10.1016/0092-8674(78)90008-9

Strickland S., Mahdavi V.

The induction of differentiation in teratocarcinoma stem cells by retinoic acid.

Cell 15:393-403(1978)

PubMed=6479493; DOI=10.1111/j.1432-0436.1984.tb01420.x

Zakany J., Burg K., Rasko I.

Spontaneous differentiation in the colonies of a nullipotent embryonal carcinoma cell line (F9).

Differentiation 27:146-151(1984)