WI-38 Human Embryonic Lung Cells

The WI-38 human diploid cell line is derived from normal embryonic lung tissue of 3 months gestation. This cell line is the first human diploid cell line used for human vaccine preparation.

Adding TNF alpha to the culture solution can promote cell growth.

Tissue and Cell Type: Normal Lung

Morphology Fibroblast like, suspended growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

87% MEM (MD0300)+10% fetal bovine serum (SD0200)++L-glutamine (SD0007)+NEAA+SP（SD0008）+G/A (SD0038)

Complete medium: MEM complete medium (article number: MD0302)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only

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Cultivation of rabies virus in human diploid cell strain WI-38.

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Hayflick L.

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Jacobs J.P.

A simple medium for the propagation and maintenance of human diploid cell strains.

Nature 210:100-101(1966)

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Glucose-6-phosphate dehydrogenase isoenzymes in human cell cultures determined by sucrose-agar gel and cellulose acetate zymograms.

Proc. Soc. Exp. Biol. Med. 128:772-776(1968)

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Stanbridge E., Onen M., Perkins F.T., Hayflick L.

Karyological and morphological characteristics of human diploid cell strain WI-38 infected with mycoplasmas.

Exp. Cell Res. 57:397-410(1969)

PubMed=4329447; DOI=10.1084/jem.133.3.454

Giraldo G., Beth E., Hirshaut Y., Aoki T., Old L.J., Boyse E.A., Chopra H.C.

Human sarcomas in culture. Foci of altered cells and a common antigen; induction of foci and antigen in human fibroblast cultures by filtrates.

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A comparison of three in vivo assays for cell tumorigenicity.

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Doubling potential, calendar time, and donor age of human diploid cells in culture.

Exp. Cell Res. 84:363-366(1974)