Hepa 1-6 mouse liver cancer cells luc

BW7756 mouse hepatoma derived from C57/L mice. The detection showed that the limb bone malformation virus (mouse pox) was negative. This cell can reproduce in serum-free medium with DMEM, 75%; Waymouth's MAB87/3 medium, 25%. Add 3x10-8M selenium. This is the labeled Luc cell line.

Luciferase is an enzyme that emits light when there is a substrate (D-luciferin) in the organism. This process is called bioluminescence. The most common luciferase gene comes from a light producing enzyme family called firefly luciferase 1 and 2.

In preclinical studies, small animal living imaging plays a vital role in visualizing physiological processes, disease progression and treatment development. The cell line activated by luciferase (luc) provides a simple, high-throughput and robust method through bioluminescence imaging (BLI) 1,2, which can be used to quantitatively evaluate the tumor burden and tumor response to treatment in test animals.

Luc cell markers have many advantages, such as:

(1) Noninvasive real-time whole-body tumor monitoring and imaging

(2) Continuous evaluation of tumor progression and treatment response in the same animal

(3) Transfer assessment

(4) Reduce the need for animal sacrifice

Tissue source/Issue and Cell Type Organ: liver strain: C57L Disease: liver cancer

Morphology epithelial cell-like, adherent growth

Medium and Culture Conditions

90% DMEM (MD0100)+10% fetal bovine serum (SD0100)+G/A (SD0038)

DMEM complete medium (article number: MD0101)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is only used for scientific research.