SK-OV-3 human ovarian cancer cells -Luc

SK-OV-3 was isolated from ascites of women with ovarian tumors by G. Trempe and L.J. Old in 1973. It is reported that this cell is resistant to tumor necrosis factor and several cytotoxic drugs including diphtheria toxin, cisplatin and adriamycin, but this library has not been verified. The tumor was induced in nude mice and formed moderately differentiated adenocarcinoma consistent with ovarian carcinoma in situ. This is the labeled Luc cell line.

Luciferase is an enzyme that emits light when there is a substrate (D-luciferin) in the organism. This process is called bioluminescence. The most common luciferase gene comes from a light producing enzyme family called firefly luciferase 1 and 2.

In preclinical studies, small animal living imaging plays a vital role in visualizing physiological processes, disease progression and treatment development. The cell line activated by luciferase (luc) provides a simple, high-throughput and robust method through bioluminescence imaging (BLI) 1,2, which can be used to quantitatively evaluate the tumor burden and tumor response to treatment in test animals.

Luc cell markers have many advantages, such as:

(1) Noninvasive real-time whole-body tumor monitoring and imaging

(2) Continuous evaluation of tumor progression and treatment response in the same animal

(3) Transfer assessment

(4) Reduce the need for animal sacrifice

Tissue source/Issue and Cell Type: ovarian adenocarcinoma, ascites metastasis

Morphology: epithelial-like, adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

90% McCoy's 5A (MD0900)+10% fetal bovine serum (SD0200)+G/A (SD0038)

Complete medium: McCOY's 5A complete medium (article No.: MD0901)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only