UACC-2727 human ovarian cancer cells

The ovarian cancer cell line UACC-2727 is derived from the greater omentum mass of a 63-year-old female without treatment. Pathologically confirmed samples were consistent with stage 4 metastatic papillary serous carcinoma, expressing her2/neu, EGFR, N-myc, MAK-6, but not estrogen receptor, progesterone receptor (PR)

Organizational source/Issue and Cell Type: Retroperitoneal metastasis of ovarian cancer

Morphology: Epithelial like, adherent growth

Complete Growth Medium and Culture Conditions

90%L-15(MD1100)+5%FBS(SD0200)+0.01 mg/ml transferrin+0.01 mg/ml insulin+5 µg/mL (55 U/ml) catalase+3.6 µg/mL (0.01 mM) hydrocortisone+70 µg/mL (0.5mM) o-phosphoethanolamine+10 ng/ml human recombinant epidermal growth factor (EGF)+3 ng/ml (0.01 µM) estradiol +0.8 ng/ml (1 pM) Na-L-thyroxine+2 mM glutamine+G/A (SD0038)

Customized complete medium: L-15 complete medium (article number: MD0000)

Gas phase: air, 100%; Carbon dioxide, 0%. Temperature: 37 ℃

Cryopreservation: basic medium+5% DMSO+20% FBS

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only

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