LLC Mouse Lung Cancer Cells

This cell line was established in the lung of C57BL mice, and may be the same cell line as 3LL cells. The tumor of this mouse originated from the implantation of primary Lewis lung cancer. The cells were resistant to 1,3-bis (2-chloroethyl) - 1-nitrosourea, but sensitive to methotrexate. It is reported that these cells have high tumorigenicity in mice, but their metastasis is weak. The cells form multiple layers in the flask without real fusion. The test showed that the mouse pox virus was negative. It was put into storage after identification without mycoplasma.

Tissue and Cell Type Lung

Morphology Mixed form, adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

90% DMEM (MD0100)+10% fetal bovine serum (SD0200)+G/A (SD0038)

DMEM complete medium (article number: MD0101)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only

PubMed=7226139; DOI=10.1016/0304-3835(80)90130-5

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PubMed=30084848; DOI=10.1038/sdata.2018.157

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Sci. Data 5:180157-180157(2018)

PubMed=31220119; DOI=10.1371/journal.pone.0218412

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Interlaboratory study to validate a STR profiling method for intraspecies identification of mouse cell lines.

PLoS ONE 14:E0218412-E0218412(2019)