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CF-1 MEF Mouse Embryonic Fibroblast
No.：CD0260
Price： ¥1900
Products Description
P3 feeder layer cells treated by radiation, 3 cells per branch × 106。 It is used for culturing hES and mES. About three T25 or one T75 culture bottles can be laid for culturing hES. It has been identified and stored without mycoplasma.
Tissue source/Issue and Cell Type: embryo
Morphology: Fibroblast-like, adherent growth
Medium and Culture Conditions
90% DMEM base (MD0100)+10% fetal bovine serum (SD0200)+1% Glutamax (SD0007)+1% NEAA+1% GA (SD0038)
Link to complete medium: DMEM complete medium (MD0104)
Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.
Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)
Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)
Note: This product is for scientific research only
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Related products
| No. | Product | specification | Price |
|---|---|---|---|
| MD0104 | DMEM (high Sugar) Complete Medium | 500ml/125ml | ￥460.00/135.00 |
| MD0100 | DMEM (high Sugar) Basic Medium | 500ml Containing Sodium Pyruvate, Hepes,/500ml Containing No Sodium Pyruvate, Hepes | ￥60.00/78.00 |
| SD0200 | Fetal Bovine Serum FBS (Advance to superior) | 500ml/100ml/25ML/10ml | ￥4960.00/1250.00/280.00/160.00 |
| SD0007 | L-glutamine (100X) | 100ml/50ml | ￥59.00/39.00 |
| SD0038 | G/A Gentamicin Amphotericin B Mixed Solution (100x) | 100ml/50ml/5ml | ￥76.00/46.00/26.00 |
| SD0002 | Free Program Cooling Cell Cryopreservation Solution (without Serum/protein) | 50ml | ￥298 |
