DR4 MEFDR4 Mouse Embryonic Fibroblasts

P3 feeder layer cells treated by radiation, 3 cells per branch × 106。 It has four kinds of resistance, about three T25 or one T75 culture bottles can be laid. The drug concentration that can be tolerated is tested as follows: G418 (neomycin): 200 μ g/ml; Puromycin: 0.4 μ g/ml; Hygromycin: 110 μ g/ml; 6-Thioguanine: 2.5 μ g/ml。 It was put into storage after identification without mycoplasma.

Animal species/Organism mice

Tissues and Cell Type of tissue source The embryos of DR4 mice from 12.5-13.5 days gestation were prepared and treated with radiation

Morphology Fibroblast-like, adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

MEF cell complete medium (100 ml): DMEM (Invitrogen, 12430) 88 ml FBS (Biochrom, S4115) 10 ml Glutamax (Invitrogen, 35050) 1 ml Non-essential Amino Acids, 100 × (Invitrogen, 11140) 1 ml

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only

PubMed=9278500; DOI=10.1093/nar/25.18.3745

Tucker K.L., Wang Y., Dausman J., Jaenisch R.

A transgenic mouse strain expressing four drug-selectable marker genes.

Nucleic Acids Res. 25:3745-3746(1997)