A-375 [A375] Human Malignant Melanoma Cells

Human malignant melanoma cell A-375 [A375] was derived from a 54-year-old woman. It is tumorigenic in nude mice.

Tissue and Cell Type: skin

Morphology: epithelial-like, adherent growth;

Culture media and additives/Complete Growth Medium and Culture Conditions:

90% DMEM (MD0100)+10% fetal bovine serum (SD0200)+G/A (SD0038)

DMEM complete medium (article number: MD0101)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: For scientific research only.

PubMed=4357758; DOI=10.1093/jnci/51.5.1417

Giard D.J., Aaronson S.A., Todaro G.J., Arnstein P., Kersey J.H., Dosik H., Parks W.P.

In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors.

J. Natl. Cancer Inst. 51:1417-1423(1973)

PubMed=327080; DOI=10.1093/jnci/59.1.221

Fogh J., Fogh J.M., Orfeo T.

One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice.

J. Natl. Cancer Inst. 59:221-226(1977)

PubMed=833871; DOI=10.1093/jnci/58.2.209

Fogh J., Wright W.C., Loveless J.D.

Absence of HeLa cell contamination in 169 cell lines derived from human tumors.

J. Natl. Cancer Inst. 58:209-214(1977)

DOI=10.1007/BF00199208

Bruggen J., Sorg C., Macher E.

Membrane associated antigens of human malignant melanoma V: Serological typing of cell lines using antisera from nonhuman primates.

Cancer Immunol. Immunother. 5:53-62(1978)

PubMed=22282976; DOI=10.1093/carcin/1.1.21

Day R.S. III, Ziolkowski C.H.J., Scudiero D.A., Meyer S.A., Mattern M.R.

Human tumor cell strains defective in the repair of alkylation damage.

Carcinogenesis 1:21-32(1980)

DOI=10.1007/BF00205883

Bruggen J., Macher E., Sorg C.

Expression of surface antigens and its relation to parameters of malignancy in human malignant melanoma.

Cancer Immunol. Immunother. 10:121-127(1981)

PubMed=6220172

Dracopoli N.C., Fogh J.

Polymorphic enzyme analysis of cultured human tumor cell lines.

J. Natl. Cancer Inst. 70:469-476(1983)

PubMed=6500159; DOI=10.1159/000163283

Gershwin M.E., Lentz D., Owens R.B.

Relationship between karyotype of tissue culture lines and tumorigenicity in nude mice.

Exp. Cell Biol. 52:361-370(1984)

PubMed=6584666; DOI=10.1093/jnci/72.4.913

Kozlowski J.M., Hart I.R., Fidler I.J., Hanna N.

A human melanoma line heterogeneous with respect to metastatic capacity in athymic nude mice.

J. Natl. Cancer Inst. 72:913-917(1984)

PubMed=3518877; DOI=10.3109/07357908609038260

Fogh J.

Human tumor lines for cancer research.

Cancer Invest. 4:157-184(1986)

PubMed=1832891; DOI=10.1016/0277-5379(91)90277-K

Hansson J., Fichtinger-Schepman A.M.J., Edgren M.R., Ringborg U.

Comparative study of two human melanoma cell lines with different sensitivities to mustine and cisplatin.

Eur. J. Cancer 27:1039-1045(1991)

PubMed=9973934; DOI=10.1016/S0165-4608(98)00122-8

Nelson M.A., Ariza M.E., Yang J.M., Thompson F.H., Taetle R., Trent J.M., Wymer J., Massey-Brown K.S., Broome-Powell M., Easton J., Lahti J.M., Kidd V.J.

Abnormalities in the p34cdc2-related PITSLRE protein kinase gene complex (CDC2L) on chromosome band 1p36 in melanoma.

Cancer Genet. Cytogenet. 108:91-99(1999)

PubMed=10497214; DOI=10.1074/jbc.274.40.28505

Ariza M.E., Broome-Powell M., Lahti J.M., Kidd V.J., Nelson M.A.

Fas-induced apoptosis in human malignant melanoma cell lines is associated with the activation of the p34(cdc2)-related PITSLRE protein kinases.

J. Biol. Chem. 274:28505-28513(1999)

PubMed=12068308; DOI=10.1038/nature00766

Davies H., Bignell G.R., Cox C., Stephens P., Edkins S., Clegg S., Teague J.W., Woffendin H., Garnett M.J., Bottomley W., Davis N., Dicks E., Ewing R., Floyd Y., Gray K., Hall S., Hawes R., Hughes J., Kosmidou V., Menzies A., Mould C., Parker A., Stevens C., Watt S., Hooper S., Wilson R., Jayatilake H., Gusterson B.A., Cooper C., Shipley J.M., Hargrave D., Pritchard-Jones K., Maitland N.J., Chenevix-Trench G., Riggins G.J., Bigner D.D., Palmieri G., Cossu A., Flanagan A.M., Nicholson A., Ho J.W.C., Leung S.Y., Yuen S.T., Weber B.L., Seigler H.F., Darrow T.L., Paterson H., Marais R., Marshall C.J., Wooster R., Stratton M.R., Futreal P.A.

Mutations of the BRAF gene in human cancer.

Nature 417:949-954(2002)

PubMed=14871852; DOI=10.1158/0008-5472.can-03-2209

Hogan K.T., Coppola M.A., Gatlin C.L., Thompson L.W., Shabanowitz J., Hunt D.F., Engelhard V.H., Ross M.M., Slingluff C.L. Jr.

Identification of novel and widely expressed cancer/testis gene isoforms that elicit spontaneous cytotoxic T-lymphocyte reactivity to melanoma.

Cancer Res. 64:1157-1163(2004)

PubMed=15009714; DOI=10.1046/j.0022-202X.2004.22243.x

Tsao H., Goel V., Wu H., Yang G., Haluska F.G.

Genetic interaction between NRAS and BRAF mutations and PTEN/MMAC1 inactivation in melanoma.

J. Invest. Dermatol. 122:337-341(2004)

PubMed=15467732; DOI=10.1038/sj.onc.1208152

Tanami H., Imoto I., Hirasawa A., Yuki Y., Sonoda I., Inoue J., Yasui K., Misawa-Furihata A., Kawakami Y., Inazawa J.

Involvement of overexpressed wild-type BRAF in the growth of malignant melanoma cell lines.

Oncogene 23:8796-8804(2004)

PubMed=17308088; DOI=10.1158/0008-5472.CAN-06-3311

Shields J.M., Thomas N.E., Cregger M., Berger A.J., Leslie M., Torrice C., Hao H., Penland S., Arbiser J.L., Scott G., Zhou T., Bar-Eli M., Bear J.E., Der C.J., Kaufmann W.K., Rimm D.L., Sharpless N.E.

Lack of extracellular signal-regulated kinase mitogen-activated protein kinase signaling shows a new type of melanoma.

Cancer Res. 67:1502-1512(2007)

PubMed=18172304; DOI=10.1158/0008-5472.CAN-07-1939

Sabatino M., Zhao Y., Voiculescu S., Monaco A., Robbins P., Karai L., Nickoloff B.J., Maio M., Selleri S., Marincola F.M., Wang E.

Conservation of genetic alterations in recurrent melanoma supports the melanoma stem cell hypothesis.

Cancer Res. 68:122-131(2008)

PubMed=19727395; DOI=10.1371/journal.pone.0006888

Wadlow R.C., Wittner B.S., Finley S.A., Bergquist H., Upadhyay R., Finn S., Loda M., Mahmood U., Ramaswamy S.

Systems-level modeling of cancer-fibroblast interaction.

PLoS ONE 4:E6888-E6888(2009)

PubMed=20164919; DOI=10.1038/nature08768

Bignell G.R., Greenman C.D., Davies H., Butler A.P., Edkins S., Andrews J.M., Buck G., Chen L., Beare D., Latimer C., Widaa S., Hinton J., Fahey C., Fu B., Swamy S., Dalgliesh G.L., Teh B.T., Deloukas P., Yang F., Campbell P.J., Futreal P.A., Stratton M.R.

Signatures of mutation and selection in the cancer genome.

Nature 463:893-898(2010)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458

Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.

A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.

Cancer Res. 70:2158-2164(2010)

PubMed=21343389; DOI=10.1158/0008-5472.CAN-10-2958

Rose A.E., Poliseno L., Wang J., Clark M., Pearlman A., Wang G., Vega Y Saenz de Miera E.C., Medicherla R., Christos P.J., Shapiro R., Pavlick A., Darvishian F., Zavadil J., Polsky D., Hernando E., Ostrer H., Osman I.

Integrative genomics identifies molecular alterations that challenge the linear model of melanoma progression.

Cancer Res. 71:2561-2571(2011)

PubMed=21424129; DOI=10.3892/or.2011.1220

Manca A., Sini M.C., Izzo F., Ascierto P.A., Tatangelo F., Botti G., Gentilcore G., Capone M., Mozzillo N., Rozzo C., Cossu A., Tanda F., Palmieri G.

Induction of arginosuccinate synthetase (ASS) expression affects the antiproliferative activity of arginine deiminase (ADI) in melanoma cells.

Oncol. Rep. 25:1495-1502(2011)

PubMed=21673604; DOI=10.1097/CMR.0b013e32834495c3

Orgaz J.L., Benguria A., Sanchez-Martinez C., Ladhani O., Volpert O.V., Jimenez B.

Changes in the gene expression profile of A375 human melanoma cells induced by overexpression of multifunctional pigment epithelium-derived factor.

Melanoma Res. 21:285-297(2011)

PubMed=22178978; DOI=10.1016/j.freeradbiomed.2011.11.019

Swalwell H., Latimer J., Haywood R.M., Birch-Machin M.A.

Investigating the role of melanin in UVA/UVB- and hydrogen peroxide-induced cellular and mitochondrial ROS production and mitochondrial DNA damage in human melanoma cells.

Free Radic. Biol. Med. 52:626-634(2012)

PubMed=22460905; DOI=10.1038/nature11003

Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.K., Yu J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L.E., Winckler W., Reich M., Li N., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.

The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.

Nature 483:603-607(2012)

PubMed=23039341; DOI=10.1186/1476-4598-11-75

Byron S.A., Loch D.C., Wellens C.L., Wortmann A., Wu J., Wang J., Nomoto K., Pollock P.M.

Sensitivity to the MEK inhibitor E6201 in melanoma cells is associated with mutant BRAF and wildtype PTEN status.

Mol. Cancer 11:75-75(2012)

PubMed=24581590; DOI=10.1016/j.jdermsci.2014.01.006

Gehrke S., Otsuka A., Huber R., Meier B., Kistowska M., Fenini G., Cheng P., Dummer R., Kerl K., Contassot E., French L.E.

Metastatic melanoma cell lines do not secrete IL-1beta but promote IL-1beta production from macrophages.

J. Dermatol. Sci. 74:167-169(2014)

PubMed=25056119; DOI=10.1158/0008-5472.CAN-14-1232-T

Watson I.R., Li L., Cabeceiras P.K., Mahdavi M., Gutschner T., Genovese G., Wang G., Fang Z., Tepper J.M., Stemke-Hale K., Tsai K.Y., Davies M.A., Mills G.B., Chin L.

The RAC1 P29S hotspot mutation in melanoma confers resistance to pharmacological inhibition of RAF.

Cancer Res. 74:4845-4852(2014)

PubMed=25960936; DOI=10.4161/21624011.2014.954893

Boegel S., Lower M., Bukur T., Sahin U., Castle J.C.

A catalog of HLA type, HLA expression, and neo-epitope candidates in human cancer cell lines.

OncoImmunology 3:E954893-E954893(2014)

PubMed=25485619; DOI=10.1038/nbt.3080

Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z., Liu H., Degenhardt J., Mayba O., Gnad F., Liu J., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.

A comprehensive transcriptional portrait of human cancer cell lines.

Nat. Biotechnol. 33:306-312(2015)

PubMed=26405815; DOI=10.1371/journal.pone.0138210

Capaldo B.J., Roller D., Axelrod M.J., Koeppel A.F., Petricoin E.F., Slingluff C.L. Jr., Weber M.J., Mackey A.J., Gioeli D., Bekiranov S.

Systems analysis of adaptive responses to MAP kinase pathway blockade in BRAF mutant melanoma.

PLoS ONE 10:E0138210-E0138210(2015)

PubMed=26673621; DOI=10.18632/oncotarget.6548

Roller D.G., Capaldo B., Bekiranov S., Mackey A.J., Conaway M.R., Petricoin E.F., Gioeli D., Weber M.J.

Combinatorial drug screening and molecular profiling reveal diverse mechanisms of intrinsic and adaptive resistance to BRAF inhibition in V600E BRAF mutant melanomas.

Oncotarget 7:2734-2753(2016)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017

Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X., Egan R.K., Liu Q., Mironenko T., Mitropoulos X., Richardson L., Wang J., Zhang T., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.

A landscape of pharmacogenomic interactions in cancer.

Cell 166:740-754(2016)

PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005

Li J., Zhao W., Akbani R., Liu W., Ju Z., Ling S., Vellano C.P., Roebuck P., Yu Q., Eterovic A.K., Byers L.A., Davies M.A., Deng W., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y., Mills G.B., Liang H.

Characterization of human cancer cell lines by reverse-phase protein arrays.

Cancer Cell 31:225-239(2017)

PubMed=29275043; DOI=10.1016/j.jprot.2017.12.013

Liberato T., Pessotti D.S., Fukushima I., Kitano E.S., Serrano S.M.T., Zelanis A.

Signatures of protein expression revealed by secretome analyses of cancer associated fibroblasts and melanoma cell lines.

J. Proteomics 174:1-8(2018)

PubMed=29492214; DOI=10.18632/oncotarget.23989

Sini M.C., Doneddu V., Paliogiannis P., Casula M., Colombino M., Manca A., Botti G., Ascierto P.A., Lissia A., Cossu A., Palmieri G.

Genetic alterations in main candidate genes during melanoma progression.

Oncotarget 9:8531-8541(2018)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747

Dutil J., Chen Z., Monteiro A.N., Teer J.K., Eschrich S.A.

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3

Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J., Gelfand E.T., Bielski C.M., Li H., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Nature 569:503-508(2019)

PubMed=31978347; DOI=10.1016/j.cell.2019.12.023

Nusinow D.P., Szpyt J., Ghandi M., Rose C.M., McDonald E.R. III, Kalocsay M., Jane-Valbuena J., Gelfand E., Schweppe D.K., Jedrychowski M., Golji J., Porter D.A., Rejtar T., Wang Y.K., Kryukov G.V., Stegmeier F., Erickson B.K., Garraway L.A., Sellers W.R., Gygi S.P.

Quantitative proteomics of the Cancer Cell Line Encyclopedia.

Cell 180:387-402.e16(2020)