293T/17 Human Embryonic Kidney Cells

293T/17 cell line is a derivative of 293T (293tsA1609neo) cell line. 293T is a highly transduced derivative of 293 cell line, which is formed by inserting the temperature sensitive gene of SV40 T-antigen. 293T cells were cloned, and pBND and pZAP vectors were detected, and a derivative strain, 293T/17, which could produce high-titer retrovirus was obtained. The cells continued to express the large T antigen of simian virus 40 (SV40), and the 17th clone was selected due to its high transfection ability. 293T/17 cells were transfected with pCRIPov - and pCRIPgag-2 vectors to obtain ANJOU 65 (see ATCC CRL-11269) cell line. ANJOU 65 cells were transfected with pCRIPgag-2 and pGPT2E vectors to obtain a house-friendly shell expression cell line BOSC 23 (see ATCC CRL-11270). ANJOU 65 cells were transfected with pCRIPAmgag vector carrying gpt resistance gene to obtain a bidirectional expression packaging cell line of Bing (see ATCC CRL-11554). It passed mycoplasma free/STR detection in this library.

Animal species/Organism Human

Tissue and Cell Type organ: kidney

Morphology epithelial cell-like, adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

90% DMEM (MD0100)+10% fetal bovine serum (SD0200)+G/A (SD0038)

DMEM complete medium (article number: MD0101)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only

PubMed=7690960; DOI=10.1073/pnas.90.18.8392

Pear W.S., Nolan G.P., Scott M.L., Baltimore D.

Production of high-titer helper-free retroviruses by transient transfection.

Proc. Natl. Acad. Sci. U.S.A. 90:8392-8396(1993)

Patent=WO1994019478A1

Pear W.S., Nolan G.P., Scott M.L., Baltimore D.

Production of high titer helper-free retroviruses by transient transfection.

Patent number WO1994019478A1, 01-Sep-1994