RKO-E6 Human Colon Cancer Transgenic Cells

RKO-E6 cell line is a colon cancer RKO cell line transfected with pCMV-E6 by liposome method. The cell contains human papillomavirus (HPV) E6 oncogene which is stably integrated and controlled by cytomegalovirus promoter. RKO-E6 cell line and its mother line RKO can be used to study the changes of cell parameters caused by p53 deletion, p53 mediated transcription and apoptosis, etc. It was identified as free of mycoplasma/STR and put into storage.

Animal species/Organism human

Tissue Cell Type colon cancer, transfected with HPV E6 inserted into pCMV

Morphology epithelial cell-like, adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

90% MEM (MD0300)+10% fetal bovine serum (SD0200)+G/A (SD0038)

Complete medium: MEM complete medium (article number: MD0301)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only

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