RKO-AS45-1 Human Colon Cancer Transgenic Cells

The open reading frame encoding GADD45 in human cDNA was cloned into the expression vector pCMV. 3, and transfected into the colon cancer cell RKO, and the RKO-AS45-1 cell line was established. The cell contains an expression vector that stably integrates the cytomegalovirus (CMV) promoter. RKO-AS45-1 cell line and its mother line RKO can be used for the study of Gadd45 on DNA repair, apoptosis and drug sensitivity. It was identified as free of mycoplasma/STR and put into storage.

Animal species/Organism human

Tissue and Cell Type colon cancer, integrated with CMV promoter of GADD45a expression vector

Morphology epithelial cell-like, adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

90% MEM (MD0300)+10% fetal bovine serum (SD0200)+G/A (SD0038)

Complete medium: MEM complete medium (article number: MD0301)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only

PubMed=7973727; DOI=10.1126/science.7973727

Smith M.L., Chen I.T., Zhan Q., Bae I., Chen C.-Y., Gilmer T.M., Kastan M.B., O'Connor P.M., Fornace A.J. Jr.

Interaction of the p53-regulated protein Gadd45 with proliferating cell nuclear antigen.

Science 266:1376-1380(1994)