MDA-MB-435S Human Melanoma Cells

MDA-MB-435S is a spindle cell, which was screened from its parent (435) in 1976. 435 was isolated from the pleural fluid of a 31-year-old female patient with metastatic breast ductal adenocarcinoma. When tubulin was stained with fluorescent dye, the parent cells showed dispersion characteristics (type II). Recently, cDNA array studies showed that the parent (MDA-MB-435) could be classified as the origin of melanoma. It was identified as free of mycoplasma/STR and put into storage.

Animal species/Organism Human

Tissues and Cell Type melanocytes are derived from tissues. Melanoma was previously thought to be breast; breast; Catheter; Ductal carcinoma; Thymic exudate

Morphology spindle-shaped, adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

90% L-15 (MD1100)+10% fetal bovine serum (SD0200)+insulin (SD0020)+G/A (SD0038)

Complete medium: L-15 complete medium (article number: MD1104)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only

DOI=10.1016/B978-0-12-333530-2.50009-5

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