HEL299 Human Embryonic Lung Fibroblasts

      The cells are derived from normal human male fetuses; Black, belonging to diploid cells, this cell line is a limited cell line with limited passage. M2 muscarinic receptor expression was downregulated after protein kinase C stimulation.

Animal species/Organism Human

Tissue and Cell Type embryonic lung, fibroblasts

Morphology Fibroblast-like, adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

90% MEM (MD0300)+10% fetal bovine serum (SD0200)+G/A (SD0038)

Complete medium: MEM complete medium (article number: MD0301)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only

PubMed=375235; DOI=10.1073/pnas.76.3.1288
Sherwin S.A., Sliski A.H., Todaro G.J.
Human melanoma cells have both nerve growth factor and nerve growth factor-specific receptors on their cell surfaces.
Proc. Natl. Acad. Sci. U.S.A. 76:1288-1292(1979)

PubMed=6254071; DOI=10.1073/pnas.77.9.5258

Todaro G.J., Fryling C., De Larco J.E.

Transforming growth factors produced by certain human tumor cells: polypeptides that interact with epidermal growth factor receptors.

Proc. Natl. Acad. Sci. U.S.A. 77:5258-5262(1980)

PubMed=7065527; DOI=10.1164/arrd.1982.125.2.222

Hay R.J., Williams C.D., Macy M.L., Lavappa K.S.

Cultured cell lines for research on pulmonary physiology available through the American Type Culture Collection.

Am. Rev. Respir. Dis. 125:222-232(1982)