CultureBoost ™ Classical Cell Culture Medium

CultureBoost ™ Classic cell culture medium:

      Consistent with our single batch of culture media and reagents

      This medium has been optimized for many cell culture experiments. This medium is prepared with 10% serum. The kit consists of 500mL Cell Systems medium and 5mL CultureBoost ™ (including cell growth factor) and 10mL Attachment Factor ™ (outer matrix reagent used to coat cell bottles). 5mL CultureBoost ™ After the foundation is added, the medium becomes a complete medium. Note: 10mL Attachment Factor ™ The coating solution for cell growth does not need to be added into the culture medium.

Specification: 500ml medium containing serum, 10mL Attachment Factor ™ Cell growth coating solution, 5mL CultureBoost ™ Cell growth factor.

CultureBoost ™ Classic cell culture medium is applicable to cells:

Certified for more than 100 human and animal cell cultures and cell lines, including:

Human:

All ACBRI and CSC primary cells

Bone marrow cells (CFU-F and erythrocyte burst culture)

Connective tissue cell

dermal fibroblasts

Endothelial cells (aorta, artery, coronary artery, blood vessel and vein)

Fetal lung cells

Embryonic and adult stem cells

Fibroblast

Glomerular mesangial cells

Immortalized/tumor-derived mesenchymal cells

Microvascular cells (brain, coronary artery, dermis, glomerulus, liver, lung and retina)

smooth muscle cell

Neovascular tumor cells

animal:

Vascular and microvascular endothelial cells of cattle and pigs

Other instructions:

          No hormone is added to the medium. The culture medium does not contain antibiotics; bacoff ® (4Z0-643) is the recommended antibiotic for all Cell Systems media. Its MIC is or higher than 2 mg/mL.

          The culture medium does not contain phenol red. Many experiments have shown that the phenol red in the cell culture medium combines with the receptors on the cell surface to make the cells react, just as the estrogen and/or inflammatory mediators (such as prostaglandin or thromboxane A2) at the drug level exist in the culture medium. In order to optimize the experimental conditions of researchers, unless customized, the normal Cell System culture medium does not contain phenol red.

And Attachment Factor ™ Better effect of using cultured cells together

In order to achieve better cell culture effect and promote cell adhesion and regeneration, CultureBoost is used ™ Please use Attachment Factor according to the requirements of the manual to culture cells in classical culture medium ™ Coated culture bottle.

Medium preservation:

Foundation: 2 - 8 ℃

5mL CultureBoost ™ Cell growth factor, 10mL Attachment Factor ™ Cell growth coating solution: - 20 ℃.

4Z0-500 culture medium is prepared by WFI. All ingredients comply with cGMP and ISO standards. The product is only used for scientific research.

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"Primary human liver co-culture with flow and Kupffer cell integration on microfluidic liver-on-a-chip" Mazur et al. (Dissertation thesis) 2015

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"Aflibercept traps Galectin-1, an angiogenic factor associated with diabetic retinopathy" Kanda et al. Scientific Reports, 2015

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SDS

4Z0-500