1.1B4 Human Islet Fusion Cells

This cell line is an electrofusion cell, which is derived from primary cultured human islet cells and PANC-1 cells, and has been applied to the study of pancreatic cell biology. It was identified as free of mycoplasma/STR and put into storage.

Animal species/Organism Human

Tissue and Cell Type islet cells, PANC-1 cells

Morphology epithelial cell-like, adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

90% 1640 (MD0200)+10% fetal bovine serum (SD0200)+150mg/L L-glutamine (SD0007)+insulin (SD0020)+5 μ g/ml Hydrocortisone+G/A (SD0038)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only

Patent=US20040005702

Flatt P.R., McClenaghan N.H.

Insulin producing cell-line.

Patent number US20040005702, 08-Jan-2004

PubMed=21515691; DOI=10.1074/jbc.M111.226795

McCluskey J.T., Hamid M., Hong G.-P., McClenaghan N.H., Gomis R., Flatt P.R.

Development and functional characterization of insulin-releasing human pancreatic beta cell lines produced by electrofusion.

J. Biol. Chem. 286:21982-21992(2011)

PubMed=23985558; DOI=10.4161/isl.26184

Vasu S., McClenaghan N.H., McCluskey J.T., Flatt P.R.

Cellular responses of novel human pancreatic beta-cell line, 1.1B4 to hyperglycemia.

Islets 5:170-177(2013)