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  • DB人B淋巴细胞
  • DB人B淋巴细胞

DB Human B Lymphocytes

No.:CD0458

Price: ¥2500.00 ¥2800.00

specification:
1E+6 Cells Flask
amount: - +
  • DB人B淋巴细胞
  • DB人B淋巴细胞
Products Description

The DB cell line was established by Walter J. Urba and Dan L. Longo from the ascites of a patient with diffuse large cell lymphoma. The cells expressed CD19+in a 45 year old white male patient; CD20 +; CD22 +; Hle-1 +; CD21 - Characterization; CD25, cells do not express HLA-DR, HLA-DQ, IgM, IgD, or T cell r antigen receptors. Exposure of DB cells to phorbol esters (PMA or PdBu) that activate protein kinase C can lead to severe growth inhibition. Their Epstein Barr virus genome is reported to be negative. After 21 days of BM-cy treatment, the cells infected with Mycoplasma were cured.



Morphology: Lymphoblasts, suspended growth

Medium and Culture Conditions:

90% 1640 (MD0200)+10% fetal bovine serum (SD0200)+G/A (SD0038)

Complete medium: 1640 Complete medium (article number: MD0201)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 degrees Celsius.

Freeze storage conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: non programmed cooling cell cryopreservation solution (low serum) (SD0001)




Note: This product is only for scientific research use


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