H209 Human Small Cell Lung Cancer Cells

This cell was isolated and established by GazdarAF and colleagues from the bone marrow metastasis of a small cell lung cancer patient in 1979. The acquisition of this bone marrow sample was prior to the treatment of the patient. This cell is a typical small cell lung cancer cell, expressing four biochemical markers at a high level: neuro-specific enol, creatine kinase brain-type isoenzyme, levodopa decarboxylase, and bombesin like immunoreactivity. The c-mycDNA sequence was not amplified; No large structural DNA abnormality was found; This cell synthesized p53 mRNA equivalent to that of normal lung. The cells grow in suspension in the form of aggregates. Only the cells in the aggregates are active, but the cell viability cannot be estimated. Generally, the medium contains a large number of cell fragments. The cell expressed abnormal RB1 protein.

Organizational source/Issue and Cell Type: Lung; Bone marrow metastases

Morphology: Round cell like, suspended cluster growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

80% 1640 (MD0200)+20% fetal bovine serum (SD0200)+G/A (SD0038)

Complete medium: 1640 complete medium (article number: MD0203)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only

PubMed=6148444; DOI=10.1093/jnci/73.4.801

Morstyn G., Russo A., Carney D.N., Karawya E., Wilson S.H., Mitchell J.B.

Heterogeneity in the radiation survival curves and biochemical properties of human lung cancer cell lines.

J. Natl. Cancer Inst. 73:801-807(1984)

PubMed=2985257

Carney D.N., Gazdar A.F., Bepler G., Guccion J.G., Marangos P.J., Moody T.W., Zweig M.H., Minna J.D.

Establishment and identification of small cell lung cancer cell lines having classic and variant features.

Cancer Res. 45:2913-2923(1985)

PubMed=2986243; DOI=10.1007/978-3-642-82372-5_4

Whang-Peng J., Lee E.C.

Cytogenetics of human small cell lung cancer.

Recent Results Cancer Res. 97:37-46(1985)

PubMed=1563005

Giaccone G., Battey J., Gazdar A.F., Oie H.K., Draoui M., Moody T.W.

Neuromedin B is present in lung cancer cell lines.

Cancer Res. 52:2732s-2736s(1992)

PubMed=7736387; DOI=10.1002/1097-0142(19950515)75:10

Campling B.G., Sarda I.R., Baer K.A., Pang S.C., Baker H.M., Lofters W.S., Flynn T.G.

Secretion of atrial natriuretic peptide and vasopressin by small cell lung cancer.

Cancer 75:2442-2451(1995)

PubMed=8626706; DOI=10.1074/jbc.271.19.11477

Quinn K.A., Treston A.M., Unsworth E.J., Miller M.-J., Vos M., Grimley C., Battey J., Mulshine J.L., Cuttitta F.

Insulin-like growth factor expression in human cancer cell lines.

J. Biol. Chem. 271:11477-11483(1996)

PubMed=8806092; DOI=10.1002/jcb.240630505

Phelps R.M., Johnson B.E., Ihde D.C., Gazdar A.F., Carbone D.P., McClintock P.R., Linnoila R.I., Matthews M.J., Bunn P.A. Jr., Carney D.N., Minna J.D., Mulshine J.L.

NCI-Navy Medical Oncology Branch cell line data base.

J. Cell. Biochem. 63 Suppl. 24:32-91(1996)

PubMed=8806103; DOI=10.1002/jcb.240630516

Johnson B.E., Russell E., Simmons A.M., Phelps R.M., Steinberg S.M., Ihde D.C., Gazdar A.F.

MYC family DNA amplification in 126 tumor cell lines from patients with small cell lung cancer.

J. Cell. Biochem. 63 Suppl. 24:210-217(1996)

PubMed=9815546

Campling B.G., Young L.C., Baer K.A., Lam Y.-M., Deeley R.G., Cole S.P.C., Gerlach J.H.

Expression of the MRP and MDR1 multidrug resistance genes in small cell lung cancer.

Clin. Cancer Res. 3:115-122(1997)

PubMed=9559344; DOI=10.1002/(SICI)1098-2264(199804)21:4

Mulligan L.M., Timmer T., Ivanchuk S.M., Campling B.G., Young L.C., Rabbitts P.H., Sundaresan V., Hofstra R.M.W., Eng C.

Investigation of the genes for RET and its ligand complex, GDNF/GFR alpha-I, in small cell lung carcinoma.

Genes Chromosomes Cancer 21:326-332(1998)

PubMed=10037197

Tang X.X., Brodeur G.M., Campling B.G., Ikegaki N.

Coexpression of transcripts encoding EPHB receptor protein tyrosine kinases and their ephrin-B ligands in human small cell lung carcinoma.

Clin. Cancer Res. 5:455-460(1999)

PubMed=10987304

Girard L., Zochbauer-Muller S., Virmani A.K., Gazdar A.F., Minna J.D.

Genome-wide allelotyping of lung cancer identifies new regions of allelic loss, differences between small cell lung cancer and non-small cell lung cancer, and loci clustering.

Cancer Res. 60:4894-4906(2000)

PubMed=11005564; DOI=10.1038/sj.neo.7900094

Kohno T., Sato T., Takakura S., Takei K., Inoue K., Nishioka M., Yokota J.

Mutation and expression of the DCC gene in human lung cancer.

Neoplasia 2:300-305(2000)

PubMed=11030152; DOI=10.1038/sj.onc.1203815

Modi S., Kubo A., Oie H., Coxon A.B., Rehmatulla A., Kaye F.J.

Protein expression of the RB-related gene family and SV40 large T antigen in mesothelioma and lung cancer.

Oncogene 19:4632-4639(2000)

PubMed=11314036; DOI=10.1038/sj.onc.1204211

Forgacs E., Wren J.D., Kamibayashi C., Kondo M., Xu X.L., Markowitz S.D., Tomlinson G.E., Muller C.Y., Gazdar A.F., Garner H.R., Minna J.D.

Searching for microsatellite mutations in coding regions in lung, breast, ovarian and colorectal cancers.

Oncogene 20:1005-1009(2001)

PubMed=14762065; DOI=10.1101/gr.2012304

Bignell G.R., Huang J., Greshock J., Watt S., Butler A., West S., Grigorova M., Jones K.W., Wei W., Stratton M.R., Futreal P.A., Weber B., Shapero M.H., Wooster R.

High-resolution analysis of DNA copy number using oligonucleotide microarrays.

Genome Res. 14:287-295(2004)

PubMed=16157194; DOI=10.1016/j.cancergencyto.2005.03.007

Grigorova M., Lyman R.C., Caldas C., Edwards P.A.W.

Chromosome abnormalities in 10 lung cancer cell lines of the NCI-H series analyzed with spectral karyotyping.

Cancer Genet. Cytogenet. 162:1-9(2005)

PubMed=17426248; DOI=10.1158/1541-7786.MCR-06-0367

Olejniczak E.T., Van Sant C., Anderson M.G., Wang G., Tahir S.K., Sauter G., Lesniewski R., Semizarov D.

Integrative genomic analysis of small-cell lung carcinoma reveals correlates of sensitivity to bcl-2 antagonists and uncovers novel chromosomal gains.

Mol. Cancer Res. 5:331-339(2007)

PubMed=19472407; DOI=10.1002/humu.21028

Blanco R., Iwakawa R., Tang M., Kohno T., Angulo B., Pio R., Montuenga L.M., Minna J.D., Yokota J., Sanchez-Cespedes M.

A gene-alteration profile of human lung cancer cell lines.

Hum. Mutat. 30:1199-1206(2009)

PubMed=20016488; DOI=10.1038/nature08629

Pleasance E.D., Stephens P.J., O'Meara S., McBride D.J., Meynert A., Jones D., Lin M.-L., Beare D., Lau K.W., Greenman C., Varela I., Nik-Zainal S., Davies H.R., Ordonez G.R., Mudie L.J., Latimer C., Edkins S., Stebbings L.A., Chen L., Jia M., Leroy C., Marshall J., Menzies A., Butler A., Teague J.W., Mangion J., Sun Y.A., McLaughlin S.F., Peckham H.E., Tsung E.F., Costa G.L., Lee C.C., Minna J.D., Gazdar A.F., Birney E., Rhodes M.D., McKernan K.J., Stratton M.R., Futreal P.A., Campbell P.J.

A small-cell lung cancer genome with complex signatures of tobacco exposure.

Nature 463:184-190(2010)

PubMed=20164919; DOI=10.1038/nature08768

Bignell G.R., Greenman C.D., Davies H., Butler A.P., Edkins S., Andrews J.M., Buck G., Chen L., Beare D., Latimer C., Widaa S., Hinton J., Fahey C., Fu B., Swamy S., Dalgliesh G.L., Teh B.T., Deloukas P., Yang F., Campbell P.J., Futreal P.A., Stratton M.R.

Signatures of mutation and selection in the cancer genome.

Nature 463:893-898(2010)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458

Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.

A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.

Cancer Res. 70:2158-2164(2010)

PubMed=20624269; DOI=10.1186/1756-9966-29-75

Du L., Schageman J.J., Irnov I., Girard L., Hammond S.M., Minna J.D., Gazdar A.F., Pertsemlidis A.

MicroRNA expression distinguishes SCLC from NSCLC lung tumor cells and suggests a possible pathological relationship between SCLCs and NSCLCs.

J. Exp. Clin. Cancer Res. 29:75-75(2010)

PubMed=22460905; DOI=10.1038/nature11003

Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.K., Yu J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L.E., Winckler W., Reich M., Li N., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.

The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.

Nature 483:603-607(2012)

PubMed=22961666; DOI=10.1158/2159-8290.CD-12-0112

Byers L.A., Wang J., Nilsson M.B., Fujimoto J., Saintigny P., Yordy J., Giri U., Peyton M., Fan Y.H., Diao L., Masrorpour F., Shen L., Liu W., Duchemann B., Tumula P., Bhardwaj V., Welsh J., Weber S., Glisson B.S., Kalhor N., Wistuba I.I., Girard L., Lippman S.M., Mills G.B., Coombes K.R., Weinstein J.N., Minna J.D., Heymach J.V.

Proteomic profiling identifies dysregulated pathways in small cell lung cancer and novel therapeutic targets including PARP1.

Cancer Discov. 2:798-811(2012)

PubMed=23716474; DOI=10.1002/gcc.22076

Iwakawa R., Takenaka M., Kohno T., Shimada Y., Totoki Y., Shibata T., Tsuta K., Nishikawa R., Noguchi M., Sato-Otsubo A., Ogawa S., Yokota J.

Genome-wide identification of genes with amplification and/or fusion in small cell lung cancer.

Genes Chromosomes Cancer 52:802-816(2013)

PubMed=25485619; DOI=10.1038/nbt.3080

Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z., Liu H., Degenhardt J., Mayba O., Gnad F., Liu J., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.

A comprehensive transcriptional portrait of human cancer cell lines.

Nat. Biotechnol. 33:306-312(2015)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017

Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X., Egan R.K., Liu Q., Mironenko T., Mitropoulos X., Richardson L., Wang J., Zhang T., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.

A landscape of pharmacogenomic interactions in cancer.

Cell 166:740-754(2016)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747

Dutil J., Chen Z., Monteiro A.N., Teer J.K., Eschrich S.A.

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3

Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J., Gelfand E.T., Bielski C.M., Li H., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Nature 569:503-508(2019)