LC-2/AD Human Lung Cancer Adenocarcinoma Cells

Establishment of lung adenocarcinoma from pleural effusion in a 51-year-old Japanese woman. LC-2/ad cells are epithelial and tend to form small round mounds. Immunocytochemical staining showed that CEA and cytokeratin were positive, including cytokeratin No.18. LC-2/ad produces at least two functional and active trypsin inhibitors and several proteases in vitro, which are identified and stored without mycoplasma/STR.

Animal species/Organism Japanese female, 51 years old

Tissues and Cell Type pleural effusion, lung adenocarcinoma

Morphology  Epithelial like, adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

45% 1640 (MD0200)+45% F12 (MD0500)+10% fetal bovine serum (SD0200)+SP (SD0008)+G/A (SD0038)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only

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