HeLa 229 Human Cervical Cancer Cells

It has certain resistance to polio virus. Keratin immunoperoxidase staining was positive. It was identified as free of mycoplasma/STR and put into storage.

Animal species/Organism people

Tissue and Cell Type cervix; Adenocarcinoma

Morphology epithelial cell-like, adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

90% 1640 (MD0200)+10% fetal bovine serum (SD0200)+G/A (SD0038)

DMEM complete medium (article number: MD0201)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only

PubMed=14185313; DOI=10.1126/science.146.3641.241

Cell Culture Collection Committee

Animal cell strains. The Cell Culture Collection Committee has assembled and certified 23 strains of animal cells.

Science 146:241-243(1964)

PubMed=2754009

Thewessen E.A.P.M., Freundt I., van Rijsoort-Vos J.H., Stolz E., Michel M.F., Wagenvoort J.H.T.

Comparison of HeLa 229 and McCoy cell cultures for detection of Chlamydia trachomatis in clinical specimens.

J. Clin. Microbiol. 27:1399-1400(1989)

PubMed=1522048; DOI=10.1007/BF02634140

Kataoka E., Honma M., Ohnishi K., Sofuni T., Mizusawa H.

Application of highly polymorphic DNA markers to the identification of HeLa cell sublines.

In Vitro Cell. Dev. Biol. Anim. 28:553-556(1992)

DOI=10.11418/jtca1981.17.3_101

Itami C., Ishida R., Utsumi K.R., Nakabayashi T.

Variation in three groups of HeLa cell sublines as revealed by karyotype analysis and O6-methylguaninedna-methyltransferase activity.

Tissue Cult. Res. Commun. 17:101-106(1998)