Hela. S3 Human Cervical Cancer Cells

The cell line was established by PuckTT, Marcus PI and CieciuraSJ in 1955 and contains HPV-18 sequence; Keratin positive; It can be used for cloning analysis of mammalian cells related to chromosome mutation, cell nutrition and colony formation.


Organizational source/Issue and Cell Type: Cervix
Morphology: Epithelial like, adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

90% F12K (MD0400)+10% neonatal bovine serum+G/A (SD0038)

Complete medium: F12K complete medium (article number: MD0401)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only

PubMed=16589695; DOI=10.1073/pnas.41.7.432

Puck T.T., Marcus P.I.

A rapid method for viable cell titration and clone production with HeLa cells in tissue culture: the use of X-irradiated cells to supply conditioning factors.

Proc. Natl. Acad. Sci. U.S.A. 41:432-437(1955)

PubMed=13286432; DOI=10.1084/jem.103.2.273

Puck T.T., Marcus P.I., Cieciura S.J.

Clonal growth of mammalian cells in vitro; growth characteristics of colonies from single HeLa cells with and without a feeder layer.

J. Exp. Med. 103:273-283(1956)

PubMed=1180926; DOI=10.1016/0006-291x(75)90551-3

Fujiwara Y., Tatsumi M.

Repair of mitomycin C damage to DNA in mammalian cells and its impairment in Fanconi's anemia cells.

Biochem. Biophys. Res. Commun. 66:592-598(1975)

PubMed=978597; DOI=10.1269/jrr.17.154

Nikaido O., Horikawa M.

Analysis of the factors in determining radiosensitivity in mammalian cells by using radio-sensitive and -resistant clones isolated from HeLa S3 cells in vitro.

J. Radiat. Res. 17:154-173(1976)

PubMed=566722; DOI=10.1007/BF02616110

Lavappa K.S.

Survey of ATCC stocks of human cell lines for HeLa contamination.

In Vitro 14:469-475(1978)

PubMed=3986962; DOI=10.1093/carcin/6.4.549

Watatani M., Ikenaga M., Hatanaka T., Kinuta M., Takai S.-I., Mori T., Kondo S.

Analysis of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)-induced DNA damage in tumor cell strains from Japanese patients and demonstration of MNNG hypersensitivity of Mer xenografts in athymic nude mice.

Carcinogenesis 6:549-553(1985)

PubMed=3756862

Maynard K., Parsons P.G.

Cross-sensitivity of methylating agents, hydroxyurea, and methotrexate in human tumor cells of the Mer- phenotype.

Cancer Res. 46:5009-5013(1986)

PubMed=3180844; DOI=10.1159/000132579

Chen T.-R.

Re-evaluation of HeLa, HeLa S3, and HEp-2 karyotypes.

Cytogenet. Cell Genet. 48:19-24(1988)

PubMed=1522048; DOI=10.1007/BF02634140

Kataoka E., Honma M., Ohnishi K., Sofuni T., Mizusawa H.

Application of highly polymorphic DNA markers to the identification of HeLa cell sublines.

In Vitro Cell. Dev. Biol. Anim. 28:553-556(1992)

CLPUB00481

Kuno H., Yoshida T.

Detection of human papillomavirus types 16, 18, and 33 in cell lines derived from human genital organs by polymerase chain reaction.

Res. Commun. Inst. Ferment. Osaka 18:6-12(1997)

PubMed=9290701; DOI=10.1002/(SICI)1098-2744(199708)19:4

Jia L.-Q., Osada M., Ishioka C., Gamo M., Ikawa S., Suzuki T., Shimodaira H., Niitani T., Kudo T., Akiyama M., Kimura N., Matsuo M., Mizusawa H., Tanaka N., Koyama H., Namba M., Kanamaru R., Kuroki T.

Screening the p53 status of human cell lines using a yeast functional assay.

Mol. Carcinog. 19:243-253(1997)

DOI=10.11418/jtca1981.17.3_101

Itami C., Ishida R., Utsumi K.R., Nakabayashi T.

Variation in three groups of HeLa cell sublines as revealed by karyotype analysis and O6-methylguaninedna-methyltransferase activity.

Tissue Cult. Res. Commun. 17:101-106(1998)

PubMed=23325432; DOI=10.1101/gr.147942.112

Varley K.E., Gertz J., Bowling K.M., Parker S.L., Reddy T.E., Pauli-Behn F., Cross M.K., Williams B.A., Stamatoyannopoulos J.A., Crawford G.E., Absher D.M., Wold B.J., Myers R.M.

Dynamic DNA methylation across diverse human cell lines and tissues.

Genome Res. 23:555-567(2013)

PubMed=23925245; DOI=10.1038/nature12064

Adey A., Burton J.N., Kitzman J.O., Hiatt J.B., Lewis A.P., Martin B.K., Qiu R., Lee C., Shendure J.

The haplotype-resolved genome and epigenome of the aneuploid HeLa cancer cell line.

Nature 500:207-211(2013)

PubMed=30175587; DOI=10.1021/acs.jproteome.8b00392

Robin T., Bairoch A., Muller M., Lisacek F., Lane L.

Large-scale reanalysis of publicly available HeLa cell proteomics data in the context of the Human Proteome Project.

J. Proteome Res. 17:4160-4170(2018)

PubMed=30778230; DOI=10.1038/s41587-019-0037-y

Liu Y., Mi Y., Mueller T., Kreibich S., Williams E.G., Van Drogen A., Borel C., Frank M., Germain P.-L., Bludau I., Mehnert M., Seifert M., Emmenlauer M., Sorg I., Bezrukov F., Bena F.S., Zhou H., Dehio C., Testa G., Saez-Rodriguez J., Antonarakis S.E., Hardt W.D., Aebersold R.

Multi-omic measurements of heterogeneity in HeLa cells across laboratories.

Nat. Biotechnol. 37:314-322(2019)