CHL Hamster Lung Cells

It is widely used in chromosome abnormality test. It was put into storage after identification without mycoplasma.

Animal species/Organism hamster

Tissue and Cell Type lung cells

Morphology fibroblast-like， Adherent growth

Medium Formulation:

90% DMEM (MD0100)+10% fetal bovine serum (SD0200)+G/A (SD0038)

DMEM complete medium (MD0101)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: No Programmed Cryopreservation Cells Solution (low Serum)(SD0001)

Declaration: This product is for scientific research only.

PubMed=5452405; DOI=10.20772/cancersci1959.61.2_161

Koyama H., Utakoji T., Ono T.

A new cell line derived from newborn Chinese hamster lung tissue.

Gann 61:161-167(1970)

PubMed=1170023; DOI=10.1016/0092-8674(75)90021-5

Miyashita K., Kakunaga T.

Isolation of heat- and cold-sensitive mutants of Chinese hamster lung cells affected in their ability to express the transformed state.

Cell 5:131-138(1975)

PubMed=688393; DOI=10.1016/0092-8674(78)90354-9

Gupta R.S., Chan D.Y.H., Siminovitch L.

Evidence for functional hemizygosity at the Emtr locus in CHO cells through segregation analysis.

Cell 14:1007-1013(1978)

PubMed=3558490; DOI=10.1002/jcp.1041300303

Gupta R.S., Dudani A.K.

Species-specific differences in the toxicity of rhodamine 123 towards cultured mammalian cells.

J. Cell. Physiol. 130:321-327(1987)

PubMed=1370240; DOI=10.1016/0027-5107(92)90036-2

Sawada M., Kitamura R., Kamataki T.

Stable expression of monkey cytochrome P-450IA1 cDNA in Chinese hamster CHL cells and its application for detection of mutagenicity of aflatoxin B1.

Mutat. Res. 265:23-29(1992)

PubMed=7512690; DOI=10.1016/0165-7992(94)90033-7

Ono T., Norimatsu M., Yoshimura H.

Induction of chromosome aberrations by pyrimethamine in cultured Chinese hamster (CHL) cells.

Mutat. Res. 323:197-201(1994)