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COS-1 Transformed Renal Cells Of African Green Monkey SV40
No.:CD0329
Price: ¥950.00
Products Description
The cell was derived from a CV-1 cell line and obtained by transfection with SV40 encoding wild-type T antigen and starting point defect mutations; A single copy of the complete early segment of the SV40 genome is integrated into the cell. The cells express T antigen and are suitable for transfection with vectors that require SV40T antigen expression; Preserve the characteristics of SV40 cytolytic growth; Support replication of temperature sensitive A209 virus at 40 ℃; Support the replication of SV40 homozygotes with early segment deletion. Due to the DNA sequence of SV40, the cell needs to be operated in a level 2 biosafety cabinet. After identification without mycoplasma, it was put into storage.
Animal species/Organism African green monkey
Tissue and Cell Type SV40 transformed renal cells
Morphology: Fibroblast-like, adherent growth
Culture media and additives/Complete Growth Medium and Culture Conditions:
90% DMEM (MD0100)+10% fetal bovine serum (SD0200)+G/A (SD0038)
DMEM complete medium (article number: MD0101)
Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.
Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)
Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)
Note: This product is for scientific research only
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