Beta TC-6 Mouse Insulinoma Islet β Cells

This cell line is derived from a pancreatic tumor (insulinoma) grown in transgenic mice. This mouse carries SV40 regulated by rat insulin II gene promoter

Pseudogene structure of early genes. Cells contain abundant insulin and small amounts of glucagon and somatostatin. Insulin is secreted in response to glucose.

Tissue and Cell Type: Pancreatic; Insulinoma

Morphology: epithelial-like, adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

85% DMEM (MD0100)+15% heat-inactivated fetal bovine serum (SD0200)+G/A (SD0038)

DMEM complete medium (article number: MD0140)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only

PubMed=7958492; DOI=10.2337/diab.43.12.1413

Knaack D., Fiore D.M., Surana M., Leiser M., Laurance M.E., Fusco-DeMane D., Hegre O.D., Fleischer N., Efrat S.

Clonal insulinoma cell line that stably maintains correct glucose responsiveness.

Diabetes 43:1413-1417(1994)

PubMed=7533732; DOI=10.2337/diab.44.3.306

Poitout V., Stout L.E., Armstrong M.B., Walseth T.F., Sorenson R.L., Robertson R.P.

Morphological and functional characterization of beta TC-6 cells -- an insulin-secreting cell line derived from transgenic mice.

Diabetes 44:306-313(1995)