PY230 cells

Py230 is an epithelial-like cell line that was isolated in 2004 from the mammary gland of an adult female mouse with adenocarcinoma. This cell line was deposited by L Ellies.

The Py230 cell line is an epithelial-like murine mammary luminal tumor cell line that has properties similar tothose of normal mouse mammary stem cells. It was derived from a mammary adenocarcinoma that spontaneously arose in a MMTV-PyMY (mouse mammary tumor virus promoter driven Polyoma middle T-antigen) transgenic C57BL/6 female mouse. This cell line carries the Polyoma virus middle T oncogene. (L Ellies, personal communication)

Py230 cells are cuboidal-shaped and grow in well-differentiated colonies at confluence in culture. (PubMed:  24368187)

This cell line forms luminal tumors and lung metastasis in vivo. (Deposit Form)

Py230 cells differentiate into single positive luminal, myoepithelial, and alveolar cells upon stimulation with lactogenic hormones. (L Ellies, personal communication)

Py230 cells express luminal-epithelial markers E-cadherin, cytokeratin 8 and cytokeratin14. Py230 cells are negative for expression of estrogen receptor, progesterone receptor and express low levels of HER2. (PubMed:  24368187) 

The Py230 cell line has a more differentiated phenotype and grows less aggressively in cell culture in comparison to the undifferenitiated and mesenchymal CRL-3280, Py8119 cells, which were also derived from a MMTV-PyMY transgenic C57BL/6 female mouse. (PubMed:  22531600)

Py230 cells, along with Py8119 cells, are a pair of murine mammary cell lines with distinct epithelial-like (Py230) or mesenchymal (Py8119) features derived from MMTV-PyMT transgene-induced mammary tumors in C57BL/6 mice that can be useful to study mammary tumorigenesis. (PubMed:  24368187) 

1.Guy CT, et al. Induction of mammary tumors by expression of polyomavirus middle T oncogene: a transgenic mouse model for metastatic disease. Mol. Cell Biol. 12: 954-961, 1992. PubMed: 1312220

2.Maglione JE, et al. Transgenic Polyoma middle-T mice model premalignant mammary disease. Cancer Res. 61: 8298-8305, 2001. PubMed: 11719463

3.Gibby K, et al. Early vascular deficits are correlated with delayed mammary tumorigenesis in the MMTV-PyMT transgenic mouse following genetic ablation of the NG2 proteoglycan. Breast Cancer Res. 14: R67, 2012. PubMed: 22531600

4.Biswas T, et al. Attenuation of TGF-β signaling supports tumor progression of a mesenchymal-like mammary tumor cell line in a syngeneic murine model. Cancer Lett 346: 129-138, 2014. PubMed: 24368187

5.Bao L, et al. Multipotent luminal mammary cancer stem cells model tumor heterogeneity. Breast Cancer Res. 17(1): 137, 2015. PubMed: 26467658