EMT6 mouse breast cancer cells

EMT6 was established by a transplantable mouse breast cancer, which occurred in BALB/cCRGL mice after implantation of proliferative mammary alveolar nodules. The resulting tumor cell line (named KHJJ) propagated in BALB/cKa mice and adapted to tissue culture after passage of the 25th generation of animals. The cell line was named EMT. EMT6 is an EMT clone isolated at Stanford University in 1971.

The EMT6 cell line can grow as a tumor in animals or in tissue culture. It is reported that the plating efficiency of tumor cells in vitro is 30%. It is reported that cells grown in tissue culture have 70% electroplating efficiency.

Organizational source/Issue and Cell Type: Breast

Morphology: Epithelial like, adherent growth

Medium and Culture Conditions

85% Waymouth's MB 752/1+15% fetal bovine serum (SD0200)+G/A (SD0038)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only

PubMed=4647494; DOI=10.1093/jnci/49.3.735

Rockwell S.C., Kallman R.F., Fajardo L.F.

Characteristics of a serially transplanted mouse mammary tumor and its tissue-culture-adapted derivative.

J. Natl. Cancer Inst. 49:735-749(1972)

PubMed=31220119; DOI=10.1371/journal.pone.0218412

Almeida J.L., Dakic A., Kindig K., Kone M., Letham D.L.D., Langdon S., Peat R., Holding-Pillai J., Hall E.M., Ladd M., Shaffer M.D., Berg H., Li J., Wigger G., Lund S., Steffen C.R., Fransway B.B., Geraghty B., Natoli M., Bauer B., Gollin S.M., Lewis D.W., Reid Y.A.

Interlaboratory study to validate a STR profiling method for intraspecies identification of mouse cell lines.

PLoS ONE 14:E0218412-E0218412(2019)