GL261 mouse glioblastoma

In the mid-1990s, an in vitro growth cell culture was established from Gl261 tumor. The tumor was induced in C57BL/6 mice by intracerebral injection of 3-methylcholanthrene in 1939, and then maintained by continuous transplantation in the homologous mouse line; It is described in the literature that cells carry TP53 and KRAS mutations

Organizational source/Issue and Cell Type: Glioma

Morphology: Fibroblast like, adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

90% DMEM (MD0100)+10% fetal bovine serum (SD0200)+G/A (SD0038)

Complete medium: DMEM complete medium (article number: MD0101)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only

PubMed=16734735; DOI=10.1111/j.1349-7006.2006.00208.x
Szatmari T., Lumniczky K., Desaknai S., Trajcevski S., Hidvegi E.J., Hamada H., Safrany G.
Detailed characterization of the mouse glioma 261 tumor model for experimental glioblastoma therapy.
Cancer Sci. 97:546-553(2006)

PubMed=23248259; DOI=10.4049/jimmunol.1201557
Ohlfest J.R., Andersen B.M., Litterman A.J., Xia J.-Z., Pennell C.A., Swier L.E., Salazar A.M., Olin M.R.
Vaccine injection site matters: qualitative and quantitative defects in CD8 T cells primed as a function of proximity to the tumor in a murine glioma model.
J. Immunol. 190:613-620(2013)

PubMed=23686484; DOI=10.4049/jimmunol.1203539
Litterman A.J., Zellmer D.M., Grinnen K.L., Hunt M.A., Dudek A.Z., Salazar A.M., Ohlfest J.R.
Profound impairment of adaptive immune responses by alkylating chemotherapy.
J. Immunol. 190:6259-6268(2013)

PubMed=25894527; DOI=10.1371/journal.pone.0121314
Bausch-Fluck D., Hofmann A., Bock T., Frei A.P., Cerciello F., Jacobs A., Moest H., Omasits U., Gundry R.L., Yoon C., Schiess R., Schmidt A., Mirkowska P., Hartlova A., Van Eyk J.E., Bourquin J.-P., Aebersold R., Boheler K.R., Zandstra P.W., Wollscheid B.
A mass spectrometric-derived cell surface protein atlas.
PLoS ONE 10:E0121314-E0121314(2015)

PubMed=30084848; DOI=10.1038/sdata.2018.157
Schuster H., Shao W.-G., Weiss T., Pedrioli P.G.A., Roth P., Weller M., Campbell D.S., Deutsch E.W., Moritz R.L., Planz O., Rammensee H.-G., Aebersold R., Caron E.
A tissue-based draft map of the murine MHC class I immunopeptidome.
Sci. Data 5:180157-180157(2018)

PubMed=30524896; DOI=10.1080/2162402X.2018.1501137
Genoud V., Marinari E., Nikolaev S.I., Castle J.C., Bukur V., Dietrich P.-Y., Okada H., Walker P.R.
Responsiveness to anti-PD-1 and anti-CTLA-4 immune checkpoint blockade in SB28 and GL261 mouse glioma models.
OncoImmunology 7:e1501137.1-e1501137.10(2018)