MC-38 Mouse colon Cancer cell -Luc

At first, it was thought to be a cell of mouse origin, but the mitochondrial genome of MCA38, the author of PubMed=25434994, was sequenced. The DNA sequence (KM820832.1) is shown as a rat sequence, but it is labeled as derived from "rat isolate 406". It can induce exocrine activity under the adrenal cortex hormone, and has high tumorigenicity. It has been identified and stored without mycoplasma.

This is the labeled Luc cell line.

Luciferase is an enzyme that emits light when there is a substrate (D-luciferin) in the organism. This process is called bioluminescence. The most common luciferase gene comes from a light producing enzyme family called firefly luciferase 1 and 2.

In preclinical studies, small animal living imaging plays a vital role in visualizing physiological processes, disease progression and treatment development. The cell line activated by luciferase (luc) provides a simple, high-throughput and robust method through bioluminescence imaging (BLI) 1,2, which can be used to quantitatively evaluate the tumor burden and tumor response to treatment in test animals.

Luc cell markers have many advantages, such as:

  (1) Noninvasive real-time whole-body tumor monitoring and imaging

  (2) Continuous evaluation of tumor progression and treatment response in the same animal

  (3) Transfer assessment

  (4) Reduce the need for animal sacrifice

Tissue and Cell Type: colon cancer

Morphology: epithelial-like, adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

90% 1640 base (MD0200)+10% FBS (SD0100)+1% GA (SD0038)

Link to complete medium: 1640 complete medium (MD0201)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only.