MC-38 Mouse Colon Cancer Cells

At first, it was thought to be a cell of mouse origin, but the mitochondrial genome of MCA38, the author of PubMed=25434994, was sequenced. The DNA sequence (KM820832.1) is shown as a rat sequence, but it is labeled as derived from "rat isolate 406". It can induce exocrine activity under the adrenal cortex hormone, and has high tumorigenicity. It has been identified and stored without mycoplasma.

Tissue and Cell Type: colon cancer

Morphology: epithelial-like, adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

90% 1640 base (MD0200)+10% FBS (SD0200)+1% GA (SD0038)

Link to complete medium: 1640 complete medium (MD0201)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only.

PubMed=1149045

Corbett T.H., Griswold D.P. Jr., Roberts B.J., Peckham J.C., Schabel F.M. Jr.

Tumor induction relationships in development of transplantable cancers of the colon in mice for chemotherapy assays, with a note on carcinogen structure.

Cancer Res. 35:2434-2439(1975)

PubMed=1255804; DOI=10.1093/jnci/56.4.871

Tan M.H., Holyoke E.D., Goldrosen M.H.

Murine colon adenocarcinomas: methods for selective culture in vitro.

J. Natl. Cancer Inst. 56:871-873(1976)

PubMed=25277546; DOI=10.1186/1471-2164-15-847

Didion J.P., Buus R.J., Naghashfar Z., Threadgill D.W., Morse H.C. III, de Villena F.P.

SNP array profiling of mouse cell lines identifies their strains of origin and reveals cross-contamination and widespread aneuploidy.

BMC Genomics 15:847-847(2014)

PubMed=25434994; DOI=10.3109/19401736.2014.974176

Zhang C.-F., Yu X.-J., Wang B.-Y., Chen H.-W., Hu C.-E.

Whole mitochondrial genome sequence of a rat colorectal cancer MCA38 cell line.

Mitochondrial DNA 27:2034-2035(2016)