HCT-116 Human Colon Cancer cells -Luc

HCT116 is one of three malignant cells isolated from male patients with colon cancer by M. Brattain et al. in 1979. Clones were formed in semi-solid agarose medium. HCT116 has tumorigenicity in nude mice without thymus and forms epithelioid tumors. This is the labeled Luc cell line.

Luciferase is an enzyme that emits light when there is a substrate (D-luciferin) in the organism. This process is called bioluminescence. The most common luciferase gene comes from a light producing enzyme family called firefly luciferase 1 and 2.

In preclinical studies, small animal living imaging plays a vital role in visualizing physiological processes, disease progression and treatment development. The cell line activated by luciferase (luc) provides a simple, high-throughput and robust method through bioluminescence imaging (BLI) 1,2, which can be used to quantitatively evaluate the tumor burden and tumor response to treatment in test animals.

Luc cell markers have many advantages, such as:

  (1) Noninvasive real-time whole-body tumor monitoring and imaging

  (2) Continuous evaluation of tumor progression and treatment response in the same animal

  (3) Transfer assessment

  (4) Reduce the need for animal sacrifice

Tissue source/Issue and Cell Type: colorectal cancer

Morphology: epithelial-like, adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

90% McCoy's 5A (MD0900)+10% fetal bovine serum (SD0200)+G/A (SD0038)

Complete medium: McCOY's 5A complete medium (article No.: MD0901)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only