U-87 Human glioma cell line -Luc

This is from 1966 to 1969 One of the cell lines constructed by Ponten and colleagues from malignant glioma (others include ATCC HTB-15, ATCC HTB-16 and ATCC HTB-17). The mycoplasma contamination was eliminated after sampling in September 1975. This is the labeled Luc cell line.

Luciferase is an enzyme that emits light when there is a substrate (D-luciferin) in the organism. This process is called bioluminescence. The most common luciferase gene comes from a light producing enzyme family called firefly luciferase 1 and 2.

In preclinical studies, small animal living imaging plays a vital role in visualizing physiological processes, disease progression and treatment development. The cell line activated by luciferase (luc) provides a simple, high-throughput and robust method through bioluminescence imaging (BLI) 1,2, which can be used to quantitatively evaluate the tumor burden and tumor response to treatment in test animals.

Luc cell markers have many advantages, such as:

(1) Noninvasive real-time whole-body tumor monitoring and imaging

(2) Continuous evaluation of tumor progression and treatment response in the same animal

(3) Transfer assessment

(4) Reduce the need for animal sacrifice

Tissue source/Issue and Cell Type: Brain astroblastoma

Morphology: epithelial-like, adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

90% MEM (MD0300) [90% minimum basic medium containing 2 mM L-glutamine and Earle's BSS Eagle adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-basic amino acid and 1.0 mM sodium pyruvate]+10% FBS (SD0200)+G/A (SD0038)

Complete medium: MEM high sugar complete medium (product number: MD0301)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only