Neuro-2a mouse neuroma cells -Luc

Neuro-2A was established by R.J. Klebe and F.H. Ruddle through spontaneous tumor of strain A white mice. It was taken from strain A mice, and the tumor mouse C1300 was purchased from Jackson Laboratory. This cell produces a large number of microtubule proteins, which play a role in the contraction system in response to the axial plasma flow in nerve cells. The detection of mouse pox virus was negative. It was put into storage after identification without mycoplasma. This is the labeled Luc cell line.

Luciferase is an enzyme that emits light when there is a substrate (D-luciferin) in the organism. This process is called bioluminescence. The most common luciferase gene comes from a light producing enzyme family called firefly luciferase 1 and 2.

In preclinical studies, small animal living imaging plays a vital role in visualizing physiological processes, disease progression and treatment development. The cell line activated by luciferase (luc) provides a simple, high-throughput and robust method through bioluminescence imaging (BLI) 1,2, which can be used to quantitatively evaluate the tumor burden and tumor response to treatment in test animals.

Luc cell markers have many advantages, such as:

(1) Noninvasive real-time whole-body tumor monitoring and imaging

(2) Continuous evaluation of tumor progression and treatment response in the same animal

(3) Transfer assessment

(4) Reduce the need for animal sacrifice

Tissue and Cell Type Brain

Morphology neurons and amoeba-like stem cells, adherent growth

Medium and Culture Conditions

90% MEM(MD0300)+10% fetal bovine serum (SD0100)+G/A (SD0038)

Complete medium: MEM complete medium (article number: MD0301)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only