C6 rat glioma cells -Luc

          Glial cell line C6 is a rat glioma clone induced by Benda et al. with N-nitrosomethyllurea, and was established after a series of in vitro culture and animal passage. When cells grow from low density to full bottle, S-100 production increases by 10 times. It was put into storage after identification without mycoplasma. This is the labeled Luc cell line.

           Luciferase is an enzyme that emits light when there is a substrate (D-luciferin) in the organism. This process is called bioluminescence. The most common luciferase gene comes from a light producing enzyme family called firefly luciferase 1 and 2.

           In preclinical studies, small animal living imaging plays a vital role in visualizing physiological processes, disease progression and treatment development. The cell line activated by luciferase (luc) provides a simple, high-throughput and robust method through bioluminescence imaging (BLI) 1,2, which can be used to quantitatively evaluate the tumor burden and tumor response to treatment in test animals.

Luc cell markers have many advantages, such as:

(1) Noninvasive real-time whole-body tumor monitoring and imaging

(2) Continuous evaluation of tumor progression and treatment response in the same animal

(3) Transfer assessment

(4) Reduce the need for animal sacrifice

Tissues and Cell Type Glioma, Brain, Glial Cells

Morphology Fibroblast-like, adherent growth

Medium and Culture Conditions

87.5% F12K (MD0400)+10% fetal bovine serum (SD0100)+2.5% HS horse serum(SD0300)+1% GA (SD0038)

Link to complete medium: F12K complete medium (MD0410)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0100)

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only