Induction lipid differentiation Medium. Adipogenic culture medium

Mesenchymal stem cell osteogenic differentiation kit is a self-developed low-serum medium for adipose-induced differentiation of cells. Using this medium to culture cells can be used to induce adipose-induced differentiation of cells from bone marrow, umbilical cord, fat and other tissues.

Medium formula:

Basic medium: 500ML(P1300), cell growth factor 5m(P2002)l, adipocyte inducible factor 5ml(P1304), fetal bovine serum: 25ml (SD0200), G/A: 5ml (SD0038)

• The procedure of inducing differentiation is simple and convenient.

• The induction efficiency of lipoblasts is high.

Induction steps:

One、 Use cell coating solution to coat the cell culture plate, and inoculate the passaged or resuscitated frozen cells on the 6-well plate with a density of 2 × 105 -3 × 105 cells/cm2 or 2 × 105 -3 × 105 cells/well, cultured in a 37 ℃, 5% CO2 incubator. When the cell convergence reaches 80% - 90%, carefully suck out the cell medium in the well, and add 2mL of cell fat differentiation medium to the 6-well plate, which is recorded as the 0 th day

Two、 Before cell induction, polylysine solution (product number: SD0042) can be used for cell coating, so that cells can better adhere to the wall.

Three、 Replace fresh cell fat differentiation medium every 1-2 days;

Note: In order to prevent the fat cells from falling off, it is recommended that after a large number of fat cell nodules appear in the induction process, the fluid change form should be changed to one and a half times a day.

Four、 During 14-21 days of induction, the cells were stained according to their morphological changes and growth.

Five、 After the induction of adipose differentiation, suck the adipose differentiation medium from the 6-well plate and rinse it with PBS for 1-2 times. Add 1 mL of cell fixation solution into each well and fix for 10~30 minutes;

Six、 Suck off the cell fixative and wash it with PBS twice. Add 2 mL of fat cell detection dye solution to each well for 3-5 min;

Seven、 Absorb the test dye solution and wash it with PBS for 2-3 times;

Eight、 Place the culture plate under the microscope to observe the staining effect

Statement: The product is for scientific research only.

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