Adult dermal fibroblast (HDF-α)

Human dermal fibroblasts were isolated from dermal tissue; The dermis, located in the deep layer of the epidermis, is connected with the subcutaneous tissue downward. The collagen fibers and elastic fibers of the dermal connective tissue are intertwined and buried in the matrix. It is widely used in the research of cell and molecular biology. This is mainly because it is a relatively easy to cultivate and well tolerated cell, which can be used for gene transfection, microinjection and other operations. It has been proved that fibroblasts in different parts are essentially different. Fibroblasts in tissues are in a dynamic balance, which affects health and the structural integrity of healed soft tissues. Cells fuse and connect with each other to form a network; The cells are in the shape of spindles or star-shaped flat distribution. The main functions of dermal fibroblasts under physiological conditions include: construction and maintenance of normal tissue morphology, synthesis and release of extracellular matrix, and timely accumulation and repair of damaged tissue after tissue injury.

Animal species/Organism Human

Tissue and Cell Type adult skin

Morphology Fibroblast-like, adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

FM fibroblast culture medium (article No.: P6001)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: primary cell cryopreservation solution (SD0001)

Statement: The product is for scientific research only