CCD-18Co (Human Colon Tissue Cells)

The cells were taken from 2.5 months old black women and began to enter the aging state at about 42 generations.

Animal species/Organism: human

Tissue and Cell Type: colon

Morphology Fibroblast-like, adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

90% MEM (MD0300)+10% fetal bovine serum (SD0200)+G/A (SD0038)

Complete medium: MEM complete medium (article number: MD0301)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

PubMed=16854228; DOI=10.1186/1476-4598-5-29
Bandres E., Cubedo E., Agirre X., Malumbres R., Zarate R., Ramirez N., Abajo A., Navarro A., Moreno I., Monzo M., Garcia-Foncillas J.
Identification by real-time PCR of 13 mature microRNAs differentially expressed in colorectal cancer and non-tumoral tissues.
Mol. Cancer 5:29-29(2006)

PubMed=23234512; DOI=10.1021/pr300898u
Segura V., Medina-Aunon J.A., Guruceaga E., Gharbi S.I., Gonzalez-Tejedo C., Sanchez del Pino M.M., Canals F., Fuentes M., Casal J.I., Martinez-Bartolome S., Elortza F., Mato J.M., Arizmendi J.M., Abian J., Oliveira E., Gil C., Vivanco F., Blanco F., Albar J.P., Corrales F.J.
Spanish human proteome project: dissection of chromosome 16.
J. Proteome Res. 12:112-122(2013)