BV-2 Mouse Microglia

It is derived from C57BL/6 mouse microglial cells, expressing nuclear v-myc and chromosome v-raf oncogenes, and expressing envgp70 antigen on the surface. It has the characteristics of phagocyte in morphology, phenotype and function. It was put into storage after identification without mycoplasma.

Tissue and Cell Type microglia

Morphology semi-adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

90% DMEM (MD0100)+10% fetal bovine serum (SD0200)+G/A (SD0038)

Complete medium: DMEM complete medium (article number: MD0101)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only

PubMed=2110186; DOI=10.1016/0165-5728(90)90073-V

Blasi E., Barluzzi R., Bocchini V., Mazzolla R., Bistoni F.

Immortalization of murine microglial cells by a v-raf/v-myc carrying retrovirus.

J. Neuroimmunol. 27:229-237(1990)

PubMed=19565166

Henn A., Lund S., Hedtjarn M., Schrattenholz A., Porzgen P., Leist M.

The suitability of BV2 cells as alternative model system for primary microglia cultures or for animal experiments examining brain inflammation.

ALTEX 26:83-94(2009)

PubMed=21961028; DOI=10.2174/1877381801002010148

Crain J.M., Watters J.J.

Estrogen and P2 purinergic receptor systems in microglia: therapeutic targets for neuroprotection.

Open Drug Discov. J. 2:148-167(2010)

PubMed=21214938; DOI=10.1186/1471-2164-12-14

Atwood B.K., Lopez J., Wager-Miller J., Mackie K., Straiker A.

Expression of G protein-coupled receptors and related proteins in HEK293, AtT20, BV2, and N18 cell lines as revealed by microarray analysis.

BMC Genomics 12:14-14(2011)

PubMed=22651808; DOI=10.1186/1742-2094-9-115

Stansley B., Post J., Hensley K.

A comparative review of cell culture systems for the study of microglial biology in Alzheimer's disease.

J. Neuroinflamm. 9:115-115(2012)

PubMed=26227174; DOI=10.1002/pmic.201400623

Woo J.-M., Han D.-H., Park J.-H., Kim S.-J., Kim Y.-S.

In-depth characterization of the secretome of mouse CNS cell lines by LC-MS/MS without prefractionation.

Proteomics 15:3617-3622(2015)

DOI=10.1007/s13273-019-00057-6

Mandal C., Yoon T., Park J.Y., Jung K.H., Chai Y.G.

Lipopolysaccharide-dependent transcriptional regulation of PU.1 in microglial cells.

Mol. Cell. Toxicol. 16:51-61(2020)